Naslov
Purification of yeast periplasmic proteins using protein adsorption to glucan

Autori
Teparić, Renata ; Mrša, Vladimir

Izvornik
Food technology and biotechnology (1330-9862) 35 (1997), 1; 29-32

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
acid phosphatase ; invertase ; protein adsorption to glucan

Sažetak
Partially purified yeast glucan was used for the one-step purification of acid phosphatase from the Saccharomyces cerevisiae cell extract, as well as for the purification of invertase from several commercially available preparations. In both cases pure enzymes, as judged by electrophoresis, were obtained by the adsorption of inpurities to glucan. The method described is simple, provides high yields, causes no significant protein denaturation and is inexpensive. It can most probably be used for other yeast extracellular proteins, as well as for proteins from other sources which have low binding potential for yeast glucan.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA