Naslov
Evaluation of Cytogenetic Damage in Workers Exposed to Microwave Radiation of GEM Radar Using New Criteria in Scoring Micronucleus Test

Autori
Gajski, Goran ; Garaj-Vrhovac, Vera ; Pažanin, Senijo ; Brumen, Vlatka

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings 12th International Congress of the International Radiation Protection Association "Strengthening Radiation Protection Worldwide" / - , 2008

Skup
12th International Congress of the International Radiation Protection Association "Strengthening Radiation Protection Worldwide"

Mjesto i datum
Buenos Aires, Argentina, 19.10.2008. - 24.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
GEM radar; occupational exposure; DNA damage; micronucleus test; nucleoplasmic bridges; nuclear buds

Sažetak
The cytokinesis-block micronucleus assay is one of the standard cytogenetic tools employed in the assessment of chromosomal damage subsequent to the exposure to genotoxic/cytotoxic agents on human cells. Within the frame of this study, the cytokinesis-block micronucleus assay was used to assess the baseline cytogenetic damage in binuclear lymphocytes in workers occupationally exposed to microwave radiation of GEM radar by virtue of measuring the frequency of micronuclei, nucleoplasmic bridges and nuclear buds. Microwave radiation of GEM radar equipment acts within microwave field of 10 µ ; W/cm2 to 10 mW/cm2 and frequency range of 1.5 GHz to 10.9 GHz that has contradictory cytogenetic effect on human cells and DNA molecule. For that reason we used new criteria in scoring micronucleus assay that allows us to measure nucleoplasmic bridges and nuclear buds in addition to micronucleus frequency for more accurate effect on possible damage on the level of the cells. Parameters of the micronucleus test were studied in workers occupationally exposed to microwave radiation of GEM radar and in corresponding unexposed control subjects. It was found that in the subjects who were occupationally exposed to microwave radiation, the levels of micronuclei increased and showed interindividual variations. In addition, new parameters that were measured ; nucleoplasmic bridges and nuclear buds, were detected in exposed group in compare to control group that did not shown this type of damage. Differences between mean group values were statistically significant (P<0.05). Our study indicates that micronucleus assay is a very useful tool in the assessment of cytogenetic damage of individuals exposed to microwave radiation of GEM radar equipment. On the basis of the micronucleus frequency and frequencies of nucleoplasmic bridges and nuclear buds, the micronucleus technique with an easy and short-term application and with an easy scoring can be used for detection of damage induced by this type of radiation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA