Naslov
Polymorphism at CAST locus and the quality of pork

Autori
Đurkin, Ivona ; Margeta, Vladimir ; Frajman, Polonca ; Kralik, Gordana ; Kušec, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress of the Croatian Society of Biochemistry and Molecular Biology with International Participation, Book of Abstracts. / - Osijek, 2008, 125-125

ISBN
978-953-95551-2-0

Skup
HDBMB 2008 Congress of the Croatian Society of Biochemistry and Molecular Biology with International Participation

Mjesto i datum
Osijek, Hrvatska, 17.09.2008. - 20.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
CAST ; polymorphism ; pig ; meat quality traits

Sažetak
The present study was performed in order to investigate a relationship between polymorphisms on calpastatin gene (CAST) and pig meat quality traits. Investigation was carried out on 29 crossbreed fatteners of unknown origin. Pigs were slaughtered at 130- 150 kg of live weight and blood samples for DNA analysis were taken at the slaughter line. The following indicators of technological, sensory meat quality and the chemical properties of meat were measured: initial pH and initial electric conductivity (measured on Longissimus dorsi muscle and on the ham 45 minutes post mortem) ; final pH and final electric conductivity (measured at the same places after 24h of cooling) ; drip loss (determined by “ bag method” ) ; colour (expressed as Hunter L*, a*, b* values) ; meat texture and the share of moisture, fat, protein and collagen (both determined on cooked sample of MLD after 24h of thawing). Genomic DNA was isolated from the blood by use of standard extraction protocol with phenol-chloroform-isoamil (25:24:21) alcohol and polymorphism at CAST locus was identified with HinfI restriction endonuclease. Statistical analysis showed that meat originating from pigs of AB genotype had the most favourable properties of sensory meat quality. As for the indicators of technological meat quality, statistically significant differences (p<0.05) were found between genotype AA and both BB and AB genotype for ultimate electric conductivity in Longissimus dorsi muscle, as well as between BB and both AA and AB genotypes for drip loss.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA