Naslov
INFLUENCE OF TNFd MICROSATELLITE POLYMORPHISM ON THE OUTCOME OF HEMATOPOIETIC STEM CELL TRANSPLANTATION

Autori
Štingl, Katarian ; Grubić, Zorana ; Čečuk-Jeličić, Esma ; Žunec, Renata ; Labar, Boris ; Serventi-Seiwerth, Ranka ; Brkljačić-Kerhin Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
22nd European Immunogenetics and Histocompatibility Conference

Mjesto i datum
Toulouse, Francuska, 01.04.2008. - 05.04.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TNFd microsatellite; HSCT

Sažetak
Proinflammatory cytokines such as TNFα are released as a consequence of conditioning regimens performed prior to the hematopoietic stem cell transplantation (HSCT) and serum levels of TNFα have been shown to correlate with occurrence of posttransplantion complications such as acute GvHD. Previous studies have found an association between TNFα production and TNFd microsatellite polymorphism. The aim of the present study was to investigate the possible correlation between this polymorphism and the survival of patients. Eighty patients who underwent HSCT were included in the analysis. All of them received a transplant from a sibling identical for HLA-A, -B, and -DRB1 loci. The analysis of the TNFd microsatellite was performed using PCR amplification with specific primers followed by electrophoresis on a polyacrylamide gel in ALFexpress sequencer with the B cell lines from 10th Workshop as controls. The distribution of alleles at TNFd locus among the patients did not differ from the results obtained for healthy individuals tested in previous studies. The predominance of TNFd4 allele (53.8% and 56.6% respectively) was observed in both groups and the frequencies of all other alleles were in agreement. The patients were divided according to the survival at day 100 post HSCT. No statistically significant difference was observed between patients who survived longer than 100 days post HSCT (N=68) and those who have not (N=12) with respect to the distribution of allele and genotype frequencies at TNFd locus as well as to the percentage of homozygous individuals. No correlation with worse survival rates was observed for the presence of alleles with higher number of repeats as previously suggested. The only marginally significant difference was found for TNFd3 allele, which was increased among patients with worse survival. This finding is in agreement with previous reports

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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