Naslov
Genotoxicity of honeybee venom in human lymphocytes using Comet assay

Autori
Gajski, Goran ; Garaj-Vrhovac, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters / Tsatsakis, Aristidis M ; Liesivuori J - Lahti : Elsevier, 2008, S104-S104

Skup
45th Congress of the European Societies of Toxicology

Mjesto i datum
Rodos, Grčka, 05.10.2008. - 08.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Bee venom; Comet assay; Genotoxicity; Human peripheral blood lymphocytes
(bee venom; comet assay; genotoxicity; human peripheral blood lymphocytes)

Sažetak
Bee venom (BV) has a broad array of therapeutic applications in traditional medicine to treat variety of diseases. It is also known that BV possesses anti-inflammatory and anticancer effect and that it can inhibit proliferation and induces apoptosis in cancer cells but there is not enough information regarding genotoxicity of BV on normal human cells. To investigate the effect of whole BV, peripheral blood human lymphocytes from healthy donor were exposed in vitro to different concentration (5, 10, 25, 50 and 100 μ g/ml) of whole BV at different exposure times (1, 6 and 24 h). Comet assay was used to evaluate the genotoxic effect of BV towards human cells. The results of the present study showed statistically significant increase in DNA damage caused in BV treated human lymphocytes compared to corresponding control cells for the tail length and tail moment. Our study has clearly shown genotoxic potential of whole BV on human peripheral blood lymphocytes in vitro determined by use of the alkaline comet assay in time and dose dependent manner. Based on the same results it is clear that whole BV induces DNA damage and has genotoxic potential on human lymphocytes. Results obtained by single cell gel electrophoresis indicate that comet assay is a useful tool for measuring DNA migration that is induced by natural venoms and the need for further genotoxic and cytogenetic researches using sensitive methods in detection of primary genome damage to establish the exact type and mechanism of DNA damage that is induced by BV.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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