Naslov
Tethering of ferredoxin : NADP+ oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL

Autori
Jurić, Snježana ; Hazler-Pilepić, Kroata ; Ana Tomašić, Ana ; Lepeduš, Hrvoje ; Jeličić, Branka ; Puthiyaveetil, Sujith ; Bionda, Tihana ; Vojta, Lea ; Allen, John F. ; Schleiff, Enrico ; Fulgosi, Hrvoje

Izvornik
Plant journal (0960-7412) 60 (2009), 5; 783-794

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
FNR; high-light; linear electron flow; retrograde signalling; rhodaneses

Sažetak
Working in tandem, two photosystems in the chloroplast thylakoid membranes produce a linear electron flow from H2O to NADP+. Final electron transfer from ferredoxin to NADP+ is accomplished by a flavoenzyme ferredoxin:NADP+ oxidoreductase (FNR). Here we describe TROL (thylakoid rhodanese-like protein), a nuclear-encoded component of thylakoid membranes that is required for tethering of FNR and sustaining efficient linear electron flow (LEF) in vascular plants. TROL consists of two distinct modules ; a centrally positioned rhodanese-like domain and a C-terminal hydrophobic FNR binding region. Analysis of Arabidopsis mutant lines indicates that in the absence of TROL relative electron transport rates at high-light intensities are severely lowered accompanied with significant increase in non-photochemical quenching (NPQ). Thus, TROL might represent a missing thylakoid membrane docking site for a complex between FNR, ferredoxin and NADP+. Such association might be necessary for maintaining photosynthetic redox poise and enhancement of the NPQ.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA