Naslov
Osteoclastogenic potential of bone marrow- and peripheral-hematopoietic cells in collagen induced arthritis

Autori
Ikić, Marina ; Lazić, Elvira ; Kuzmac, Sania ; Cvija, Hrvoje ; Marušić, Ana ; Grčević, Danka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Bone, 36th European Symposium on Calcified Tissues / - , 2009, S334-S334

Skup
36th European Symposium on Calcified Tissues

Mjesto i datum
Beč, Austrija, 23.05.2009. - 27.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
collagen induced arthritis; rheumatoid arthritis; osteoclast; cytokines

Sažetak
Background/aims: Collagen induced arthritis (CIA) is a mouse model for human rheumatoid arthritis (RA) and is a useful model for studying the pathogenesis of inflammatory joint disease. Our aim was to analyze osteoclastogenic potential of the bone marrow and peripheral hematopoietic cells in CIA. Methods: C57BL/6 mice were immunized with chicken type II collagen emulsified in complete Freund's adjuvant (FA), followed by a booster dose of collagen emulsified in incomplete FA at day 21. Mice were clinically scored for up to 70 days. Hematopoietic cells from different sources (bone marrow, homogenized bone shafts, spleen, or peripheral blood) were cultured with RANKL (40 ng/mL) and M-CSF (15 ng/mL) to stimulate osteoclast (OCL) differentiation. OCLs were detected as TRAP-positive multinucleated cells with 3 or more nuclei per cell. For flow-cytometry, a series of hematopoietic markers were used to characterize osteoclast progenitor (B220, CD3, NK1.1, CD115, CD117, CD11b), myeloid (NK1.1, F4/80, CD11c, CD11b) and lymphoid (CD3, CD4, CD8, B220) populations. Gene expression analysis for inflammatory, osteoclast and osteoblast specific genes was performed by qPCR. Results: The number of differentiated OCLs from peripheral blood and spleen cells was significantly higher (p<0.01) in CIA (488&plusmn ; 128 and 1917&plusmn ; 157, respectively) than in control mice (309&plusmn ; 120 and 488&plusmn ; 129, respectively). There was no significant difference in OCL numbers from bone marrow- and bone shaft-derived cells. Flow cytometry showed more CD115+ cells within both CD11b-/low and CD11b+ populations in the periphery, indicating increased number of osteoclast progenitors in CIA compared with control mice. In addition, myeloid and B-lymphoid populations increased whereas NK cell population decreased in CIA. Gene expression analysis showed increased expression of IL-17 and cFms in blood cells and decreased expression of Runx2 in bone tissue. Conclusions: Our results indicate that mice with CIA have higher number of OCL progenitors within peripheral hematopoietic cells and greater osteoclastogenic potential but similar osteoclastogenic potential of bone-marrow cells compared with control mice. We will further analyze osteoclast progenitors and bone metabolism in CIA, which may help to better understand the pathogenesis of bone resorption in RA.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA