Naslov
Expression of mRNAs exons for sterol regulatory binding proteins in mouse testis and liver.

Autori
Kalanj-Bognar, Svjetlana ; Rozman, Damjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Silver Jubilee Meeting of the Croatian Biochemical Society, Book of Abstracts / Floegel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000

Skup
Silver Jubilee Meeting of the Croatian Biochemical Society

Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Sažetak
Sterol regulatory binding proteins (SREBPs) are membrane-bound transcription factors which control the metabolism of cholesterol and fatty acids in animal cells. Two genes, designated SREBP-1 and SREBP-2, encode SREBPs. The SREBP-1 gene gives rise to two transcripts - SREBP-1a and -1c. Cleavage of SREBPs and release of transcriptional fragments which enter the nucleus and bind to sterol regulatory elements (SRE) is enhanced by sterol depletion and inhibited by sterol supplementation. Previous studies suggested that SREBP-dependent pathway is responsible for regulation of cholesterol and fatty acids biosynthesis in liver and other somatic tissues, while cAMP/cAMP-responsive element modulator CREMt-dependent regulation of cholesterogenic gene CYP51 may predominate in male germ cells. The aim of this study was to determine the expression of exons 1a, 1c and 2 in mRNAs for SREBPs in mouse germ cells (prepubertal and adult), interstitial cells, testis and liver. The analysis was performed by RT-PCR method using specific fluorescent-labeled primers for SREBP-1a, -1c and -2, and actin primer as internal standard. RT-PCR products were analyzed by capillary electrophoresis on Abi Prism Genetic Analyzer. The results showed the presence of SREBP-1c transcript in all analyzed samples (mouse liver, testis, germ and interstitial cells), while SREBP-1a transcript was not detected in any sample. SREBP-2 transcript was expressed in mouse testis, interstitial cells, and germ cells from both prepubertal and adult animal. In conclusion, the role of both sterol/SREBP signalling pathway and cAMP/CREMt dependent regulation of cholesterogenic genes in testis has to be further clarified.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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