Naslov
Specific Bone Turnover Features in Patients with Urolithiasis

Autori
Smolić, Martina ; Smolić, Robert ; Ines Bilić-Čurčić ; Opačak-Bernardi, Teuta ; Marić, Ivana ; Kizivat, Tomislav ; Mihaljević, Ivan ; Milas-Ahić, Jasminka ; Kuveždić, Hrvoje ; Tucak, Antun

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Urology Meetings ; u: European Urology (2008) (S) / Marberger, Michael - Arnhem : Elsevier, 2008, 134-134

Skup
EAU Central European Meeting (8 ; 2008)

Mjesto i datum
Varšava, Poljska, 24.10.2008. - 25.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bone ; osteopontin ; kidney stones ; osteoprotegerin

Sažetak
Introduction & Objectives: Several clinical studies revealed increased bone turnover and lower bone mass in patients with urolithiasis. Bone mass loss is particularly evident in idiopathic calcium stone formers. However, pathogenetic mechanisms and factors implicated in bone loss in these patients are still unknown. Osteopontin (OPN) is a phosphorylated protein of wide tissue distribution, that is found in association with organic matrix of kidney stones. It is a strong inhibitor of crystal formation and growth in vitro, but there is still debate regarding its effects upon crystal adhesion to tubular epithelial cells. OPN influences bone turnover, both by promoting differentiation of osteoclasts and by enhancing osteoclasts activity. Recent studies discovered important role of osteoprotegerin (OPG) and RANKL (receptor activator of NF-kappaB ligand) in bone turnover process, in addition to known effect of some cytokines (such as IL-1, IL-6, TNF) on bone resorption. The objective of this study is to assess changes of cytokines involved in bone turnover and increased bone resorption, as that might play role in bone loss in recurrent calcium stone formers. Material & Methods: Recurrent calcium stone formers were involved in the study and anthropometrical measurements were performed. Ca2+, Mg2+, P, creatinine, Na+, K+, Cl-, proteins, uric acid, alkaline phosphatase, osteocalcin, calcitonin, PTH, 25-OH D vitamin and 1, 25-OH D vitamin were assesed by standard biochemical analysis. IL-1, IL-6, TNF-α , OPG, RANKL and osteopontin serum levels were determined by quantitive enzyme-linked immunosorbent assay (ELISA) according to manufacture’ s protocol (Quantikine, R&D Systems, Inc., Minneapolis, USA). Bone mineral density (BMD ; g/cm2) was measured using dual-energy x-ray absorptiometry (« Lunar Prodigy» ). BMD measurements were performed at the lumbar spine, femoral neck, forearm and total body. Results are presented as bone mineral content (BMC ; g) and bone area (BA ; cm2) Results: Osteopenia was discovered in 27, 8% of recurrent calcium stone formers and 5, 7% had osteoporosis assessed by BMD described above. Kalcitonin and osteokalcin levels were normal in all participants. Vitamin D25 levels were increased in 16, 7% patients with a peak of 213 pmol/ml. PTH ranged from 31, 4 to 131 pg/ml and was increased in 26, 3% of patients. Pyrilinks D was increased in 30, 77% of male participants, whereas in females was increased in 57, 10%. However, crosslaps was increased in 46, 2% of male participants with no significant change in pre- and postmenopausal women. Conclusions: Increased production of cytokines enhances bone resorption and therefore lowers bone mass in patients with idiopathic calcium urolithiasis. Here we have determined factors that could have influenced the changes in bone density in recurrent calcium stone formers, but pathogenetic mechanisms still remain to be evaluated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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