Naslov
Igf2 and H19 gene polymorphisms in couples with recurrent spontaneous abortion

Autori
Pereza, Nina ; Ostojić, Saša ; Volk, Marija ; Kapović, Miljenko ; Peterlin, Borut

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the Xth International Congress of Reproductive Immunology ; u: American Journal Of Reproductive Immunology, 57 (2007) (6) 391–476) ; ICRI-07-2-92 / - , 2007, 470-470

Skup
International Congress of Reproductive Immunology (10 ; 2007)

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
recurrent spontaneous abortion susceptibility; genetic polymorphisms; Igf2; H19

Sažetak
Genetic markers could be factors of predisposition to idiopathic recurrent spontaneous abortion (RSA). Igf2 and H19 genes undergo the phenomenon of genomic imprinting, Igf2 being expressed from paternal, and H19 only from maternal allele. Igf2 is a major fetal growth factor that stimulates angiogenesis, proliferation and transport of nutrients in placenta. H19 is a non coding gene whose untranslated RNA supresses growth by controlling the level of Igf2 gene expression and Igf2 mRNA cytoplasmic localization. The aim of this study is to evaluate the association between Igf2 and H19 gene polymorphisms and the susceptibility to RSA. A case-control study was conducted to determine the association between Igf2 and H19 gene polymorphisms and the risk of RSA in 113 couples with RSA, and 113 fertile couples as control. PCR was performed to analyse DNA for Igf2 ApaI polymorphism in exon 9 and H19 HhaI polymorphism in the 6th CTCF binding site. Statistically significant difference was found for Igf2 ApaI polymorphism in male partners of RSA women. Their genotype frequencies compared with controls were: 13%/56%(AA) ; 39%/20%(GG) ; 48%/25%(AG) ; when following dominant model for risk genotypes (AA+AG), OR=0.38, p=0.001. Conclusion: Considering that Igf2 gene is expressed only from paternal allele, the ApaI polymorphism in partners of RSA women could affect Igf2 level of expression in placenta and lead to RSA. The research of polymorphisms in genes undergoing the phenomenon of genomic imprinting and including male partners, represents a new approach in the research of genetic etiology of idiopathic RSA.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Doi: 10.1111/j.1600-0897.2007.00495.x

POVEZANOST RADA