Naslov
Activation of MAPK by potassium bisperoxo(1, 10-phenantroline)oxovanadate (V)

Autori
Cerovac, Željka ; Ban, Jasna ; Morinville, Anne ; Yaccato, Karin ; Shaver, Alan ; Maysinger, Dušica

Izvornik
Neurochemistry international (0197-0186) 34 (1999), 4; 337-344

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
PC12 cells; 51V-NMR; peroxovanadium complexes; JNK; ERK

Sažetak
Potassium bisperoxo(1, 10-phenantroline)oxovanadate (V) [bpV(phen)] is a potent proteine phosphatase inhibitor which mediates a variety of biological effects. The aim of these studies was to examine the role(s) of mitogen activated protein kinase (MAPK) pathways in PC12 cell proliferation and toxicity by bpV(phen). BpV(phen) exerts a biomodal effect in PC12 cells: proliferation at low and cell death at higher micromolar concentrations. Activation of MAPK by bpV(phen) depends on time and concentration. The phosphorylation pattern of extracelllular regulated kinases (ERK ˝), c-jun N-terminal activated kinases (JNK) and p-38 in PC12 cells is strikingly different. Activation of JNK is sustained in PC12 cells. In contrast, ERK 1/2 activation is transient and treatment with PD98059 indicates that ERK activation by bpV(phen) is partly independent from the ras-MEK pathway. Stability studies of bpV(phen) in DMEM and PBS showed linear relationship with T1/2 about 6 h and 10 days in DMEM and PBS, respectively. Comparison between the time courses of MAPK activation and kinetics of bpV(phen) decomposition as assessed by 51V-NMR analysis show that the initial and maximal phosphorylation signals are produced in the presence of the complex bpV(phen) and not caused by the decomposition products of bpV(phen).

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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