Pregled bibliografske jedinice broj: 402550
Flexibility profiling of gene transcription start and end sites on human and fruit fly DNA
Flexibility profiling of gene transcription start and end sites on human and fruit fly DNA // 50 godina molekularne biologije u Hrvatskoj
Zagreb, Hrvatska, 2008. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 402550 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Flexibility profiling of gene transcription start and end sites on human and fruit fly DNA
Autori
Kokan, Tina ; Karlić, Rosa ; Vlahoviček, Kristian
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
50 godina molekularne biologije u Hrvatskoj
/ - , 2008
Skup
50 godina molekularne biologije u Hrvatskoj
Mjesto i datum
Zagreb, Hrvatska, 20.11.2008. - 21.11.2008
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
DNA; curvature; bendability; transcription
Sažetak
Flexibility of a DNA molecule plays a significant role in some important biological processes such as transcription and chromosome packaging. Curvature and bendability are sequence-dependent intrinsic properties of the DNA double helix where curvature is a measure of torsional flexibility, and bendability is a measure of anisotropic bending flexibility of a DNA molecule. We wanted to see if such a structure-encoded signal exists near transcription start (TSS) and end sites (TES) of fruit fly (D. melanogaster) and human transcripts. Using a program with an implemented BEND algorithm (1), developed inside our Bioinfo group, we managed to make profiles of DNA sequences by appending a curvature and bendability value to each nucleotide. We created curvature and bendability profiles for human and fruit fly transcripts. Fruit fly transcripts were grouped by promoter modules (2) and by a k-means algorithm. Our preliminary results show that curvature and bendability are significantly altered in close proximity to TSSs and TESs in both human and fruit fly, suggesting that the curving and bending of the sequence may have a specific function in regulation of initiationand termination of transcription. (1) Goodsell, D. S., Dickerson, R. E. 1994. Bending and curvature calculations in B-DNA. Nucleic Acids Research 22 (24), 5497-5503. (2) Ohler, U., Liao, G., Niemann, H., Rubin, G. M. 2002. Computational analysis of core promoters in the Drosophila genome. Genome Biology 3 (12), research0087.1-0087.12.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
119-0982913-1211 - Računalna genomika mikrobnih okoliša i bioinformatika ekstremofila (Vlahoviček, Kristian, MZOS ) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
