Naslov
Computational analysis of exonic splicing regulators

Autori
Stojanović, Ozren

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
03.07

Godina
2008

Stranica
38

Mentor
Vlahoviček, Kristian

Ključne riječi
splicing; ESE; splicing regulators; SR proteins

Sažetak
In the process of pre-mRNA splicing in mammals, exon recognition by spliceosome is assisted by members of serine-arginine (SR) family of proteins, which specifically bind certain short regions in the exons, called exon splicing enhancers (ESEs). Loss of an exonic regulator can lead to skipping of that exon and formation of truncated protein. Degeneracy and context-dependence of these regulators makes biological evaluation of ESEs predicted by either of two existing in silico tools very difficult. I created ESE Analyzer Web Server for evaluation of predicted ESEs based on the alignments of human paralogous sequences and observation of ESEs conservation patterns, with special regard to competing selection pressures to protein versus enhancer sequences. Further, I performed extensive statistical analysis to discover the possible patterns regarding enhancers’ distribution in the human exons. I found no preferential density, position or distance between individual or clustered enhancers. I compared these parameters to the properties of the corresponding exons (length, splice site strengths) and detected no significant correlation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Računarstvo

POVEZANOST RADA