Naslov
Cytotoxicity of propolis and its polyphenolic compounds primary culture of human urinary bladder transitional cell carcinoma

Autori
Oršolić, Nada ; Štajcar, Damir ; Filipan, Zoran ; Benčić, Krunoslav ; Benko, Goran ; Dujmović, Tonči ; Lodeta, Branimir ; Bašić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 7th EAU Central European Meeting : abstracts / - , 2007

Skup
EAU Central European Meeting (7 ; 2007)

Mjesto i datum
Zagreb, Hrvatska, 26.10.2007. - 27.10.2007

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Propolis; Flavonoids; Human urinary bladder cancer; Cytotoxicity; Primary culture

Sažetak
This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder transitional cell carcinoma (T.C.C.) cells as compared to normal urinary bladder epithelial cells. Pieces of TCC or normal epithelial tissue were collected by transurethral surgery from patients in different stages (grade G1, G2, G3) of TCC. Incubation of TCC cells for cytotoxicity testing were carried out with or without diffrent concentration (50, 150, 300 µ ; g/ml) of test components. The cytotoxicity of two preparation of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, quercetin) was determined using trypan blue exlusion assay. Findings suggest that EEP is the most effective in inhibition of urinary bladder TCC cell proliferation as compared to WSDP or single flavonoids derived from propolis. All test components showed no cytotoxic effect on normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to impruve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation or its polyphenolic components have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis effectivness have to be elucidated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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