Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 400243

Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis

Almeida, Reinaldo; Mosoarca, Cristina; Chirita, Marius; Udrescu, Valentina; Dinca, Nicolae; Vukelić, Željka; Allen, Mark; Zamfir, Alina D.
Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis // Analytical biochemistry, 378 (2008), 1; 43-52 doi:10.1016/j.ab.2008.03.039 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 400243 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis

Autori
Almeida, Reinaldo ; Mosoarca, Cristina ; Chirita, Marius ; Udrescu, Valentina ; Dinca, Nicolae ; Vukelić, Željka ; Allen, Mark ; Zamfir, Alina D.

Izvornik
Analytical biochemistry (0003-2697) 378 (2008), 1; 43-52

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
fully automated chip-nanoESI; high-capacity ion trap mass spectrometry; multistage CID; structural analysis; brain gangliosides; anencephaly

Sažetak
NanoMate robot was coupled to a high-capacity ion trap (HCT) mass spectrometer to create a system merging automatic chip-based electrospray ionization (ESI) infusion, ultrafast ion detection, and multistage sequencing at superior sensitivity. The interface between the NanoMate and HCT mass spectrometer consists of an in-laboratory constructed mounting device that allows adjustment of the robot position with respect to the mass spectrometer inlet. The coupling was optimized for ganglioside (GG) high-throughput analysis in the negative ion mode and was implemented in clinical glycolipidomics for identification and structural characterization of anencephaly-associated species. By NanoMate HCT mass spectrometry (MS), data corroborating significant differences in GG expression in anencephalic versus age-matched normal brain tissue were collected. The feasibility of chip-based nanoESI HCT multistage collision-induced dissociation (CID MS(n)) for polysialylated GG fragmentation and isomer discrimination was tested on a GT1 (d18:1/18:0) anencephaly-associated structure. MS(2)-MS(4) obtained by accumulating scans at variable fragmentation amplitudes gave rise to the first fragmentation patterns from which the presence of GT1b structural isomer could be determined unequivocally without the need for supplementary investigation by any other analytical or biochemical methods.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA

Projekti:
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Vukelić, Željka, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Željka Vukelić (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Almeida, Reinaldo; Mosoarca, Cristina; Chirita, Marius; Udrescu, Valentina; Dinca, Nicolae; Vukelić, Željka; Allen, Mark; Zamfir, Alina D.
Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis // Analytical biochemistry, 378 (2008), 1; 43-52 doi:10.1016/j.ab.2008.03.039 (međunarodna recenzija, članak, znanstveni)
Almeida, R., Mosoarca, C., Chirita, M., Udrescu, V., Dinca, N., Vukelić, Ž., Allen, M. & Zamfir, A. (2008) Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis. Analytical biochemistry, 378 (1), 43-52 doi:10.1016/j.ab.2008.03.039.
@article{article, author = {Almeida, Reinaldo and Mosoarca, Cristina and Chirita, Marius and Udrescu, Valentina and Dinca, Nicolae and Vukeli\'{c}, \v{Z}eljka and Allen, Mark and Zamfir, Alina D.}, year = {2008}, pages = {43-52}, DOI = {10.1016/j.ab.2008.03.039}, keywords = {fully automated chip-nanoESI, high-capacity ion trap mass spectrometry, multistage CID, structural analysis, brain gangliosides, anencephaly}, journal = {Analytical biochemistry}, doi = {10.1016/j.ab.2008.03.039}, volume = {378}, number = {1}, issn = {0003-2697}, title = {Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis}, keyword = {fully automated chip-nanoESI, high-capacity ion trap mass spectrometry, multistage CID, structural analysis, brain gangliosides, anencephaly} }
@article{article, author = {Almeida, Reinaldo and Mosoarca, Cristina and Chirita, Marius and Udrescu, Valentina and Dinca, Nicolae and Vukeli\'{c}, \v{Z}eljka and Allen, Mark and Zamfir, Alina D.}, year = {2008}, pages = {43-52}, DOI = {10.1016/j.ab.2008.03.039}, keywords = {fully automated chip-nanoESI, high-capacity ion trap mass spectrometry, multistage CID, structural analysis, brain gangliosides, anencephaly}, journal = {Analytical biochemistry}, doi = {10.1016/j.ab.2008.03.039}, volume = {378}, number = {1}, issn = {0003-2697}, title = {Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis}, keyword = {fully automated chip-nanoESI, high-capacity ion trap mass spectrometry, multistage CID, structural analysis, brain gangliosides, anencephaly} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Citati:

    Altmetrijski pokazatelji:


    Podijeli:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font
    CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.