Naslov
Coimmobilization of pyranose 2-oxidase, laccase and catalase on solid supports for enhanced production of 2-keto aldoses

Autori
Ludwig, Roland ; Sukyai, Prakit ; Rezić, Tonči ; Haltrich, Dietmar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Chemical Society, 234th National meeting of the American Chemical Society / - Boston (MA) : American Chemical Society (ACS), 2007

Skup
234th National meeting of the American Chemical Society

Mjesto i datum
Boston (MA), Sjedinjene Američke Države, 19.08.2007. - 23.08.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pyranose 2-oxidase; laccase; catalase; co-immobilization; 2-ketoglucose

Sažetak
The tri-enzyme system pyranose 2-oxidase (P2O), laccase, and catalase was chosen to study the most influential parameters in the homogeneous and heterogeneous application of a complex redox machinery. P2O, which reduces oxygen or alternative electron acceptors and oxidizes aldoses to the corresponding 2-keto-sugars, was immobilized on eleven agarose or acrylic resins using varying coupling methods and the binding capacity was determined. An acrylic carrier (Amberzyme Oxirane) with the most suitable properties was selected for further testing. To increase the reaction rate, laccase was introduced to provide a competing electron acceptor, benzoquinone, which shows with P2O a three-fold higher turnover number than oxygen. Catalase was used to eliminate residual hydrogen peroxide and applied in different amounts to determine the influence of the hydrogen peroxide concentration on the turnover stability.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA