Naslov
Production of paramylon, a beta-1, 3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium

Autori
Šantek, Božidar ; Felski, Michael ; Frichs, Karl ; Lotz, Martin ; Flaschel, Erwin

Izvornik
Engineering in life sciences (1618-0240) 9 (2009), 1; 23-28

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
batch cultivation; Euglena gracilis; heterotrophic growth; synthetic medium

Sažetak
Euglena gracilis was cultivated on a synthetic medium of well-defined components. Biomass and paramylon (beta-1, 3-glucan) concentrations were the most important variables monitored. Mass production in the bioreactor was carried out following studies of operating conditions in shaken flasks. E. gracilis grew best at about 30 ^oC and at a low pH of 3. A pH control was not necessary, although the pH increased considerably at the end of the cultivation processes. Aeration could be performed at low stirrer frequency. Biomass concentrations of about 13– 14 g/L were obtained with paramylon mass fractions of 50– 60%, by starting with a synthetic medium containing 15 g/L of glucose as the main carbon source.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA