Pregled bibliografske jedinice broj: 39861
Detection of Autoantibodies against Advanced Glycation Endproducts and AGE-Immune Complexes
Detection of Autoantibodies against Advanced Glycation Endproducts and AGE-Immune Complexes // Knjiga sažetaka 25.kongresa hrvatskih biokemičara i molekularnih biologa / Flogel, Mirna (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000. str. 103-103 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 39861 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Detection of Autoantibodies against Advanced Glycation Endproducts and AGE-Immune Complexes
Autori
Turk, Zdenka ; Turk, Nikša ; Benko, Bojan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Knjiga sažetaka 25.kongresa hrvatskih biokemičara i molekularnih biologa
/ Flogel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000, 103-103
Skup
25 Kongres hrvatskih biokemičara i molekularnih biologa
Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Advanced glycation endproduct; Autoantibody
Sažetak
Advanced glycation of protein causes their immunogenicity. The evidence that AGEs have antigenic properties have led to a hypothesis that the AGE structure found in vivo may exert an autoimmune response. In the present study, we showed the sera of diabetic patients as well as of nondiabetic individuals to contain autoantibodies to epitopes of AGE structures. Contrary to what might be expected, we observed lower AGE antibody titers in diabetic subjects, and postulated the antibodies against AGEs to form immune complexes in vivo, hampering their determination. The existence of immune complexes containing AGE moiety was established by two independent criteria: (a) serum AGE-immune complexes (AGE-IC) were detected by ELISA using an immunochemical bridge, and (b) soluble AGE-IC were precipitated from serum by polyethylene glycol and analysed. We demonstrated the presence of circulating AGE-IC in sera, predominantly in the sera of diabetic subjects. We also found an inverse correlation between serum AGE level and AGE-IC (r=-0.8, p<0.000), indicating the serum level of AGEs to decline with an increasing presence of AGE-IC. The content of AGE in soluble immune complexes was significantly higher in diabetic patients than in control subjects (3.51 1.9 vs 1.89 1.0  gEq/ml (p<0.00004), and correlated inversely with free antibodies (r=-0.26, p<0.01). Interactions of AGE autoantibodies with AGE as an antigen continuously produced result in the formation of AGE-immune complexes that may play a role in the atherogenic processes.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
045003
Ustanove:
Klinika za dijabetes, endokrinologiju i bolesti metabolizma Vuk Vrhovac
