Naslov
Autoantibodies against advanced glycation endproducts and AGE-immune complexes in NIDDM patient sera

Autori
Turk, Zdenka ; Ljubić, Spomenka ; Benko, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 36th Annual meeting of the European Association for the Study of Diabetes (EASD) ; u: Diabetelogia 43 (2000) (S) / Waldhauser, W. - Heidelberg : Springer, 2000, A53-A53

Skup
Annual meeting of the European Association for the Study of Diabetes (36 ; 2000))

Mjesto i datum
Jeruzalem, Izrael, 17.09.2000. - 21.09.2000

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
diabetes; advanced glycation; autoantibodies; immune complexes

Sažetak
Advanced glycation of protein results in its immunogenicity. Autoantibodies to epitopes of AGE structures were determined in the sera of NIDDM (n=64) and age-matched non-diabetic control subjects (n=20). The presence of immune complexes involving AGE as the antigenic moiety (AGE-IC) was detected in the same serum samples. A blocking ELISA for the measurement of anti-AGE autoantibodies was developed. Serum AGE- immune complexes were detected by ELISA using an immunochemical bridge. Soluble AGE-IC were precipitated from serum by polyethylenglycol and analysed. Competitive ELISA was measured total serum AGEs. The autoantibody titer, expressed as percent of inhibition, was higher in the control group than in NIDDM patients (47.3 14.4 vs 26.0 11.8 %, p<0.001). To clarify whether AGE-autoantibodies are masked in vivo by immune complexes formation we determine immune complexes containing AGE as antigen. Circulating AGE-IC showed an inverse correlation with AGE level (r=-0.8, p<0.000). The content of AGE in soluble immune complexes was significantly higher in diabetic patients than in control subjects (3.51 1.9 vs 1.89 1.0  gEq/ml (p<0.00004), and correlate inversly with anti-AGE antibodies (r=-0.26, p<0.01). AGE autoantibody negativity was defined as a value lower than the mean of controls for 2SD. Antibody negative patients (48%) had a higher level of serum AGEs (14.4 6.2 vs 13.4 6.2  gEq/ml, p<0.001), longer diabetes duration (11 5 vs 9.9 6 yrs, p<0.01) and higher urinary albumin excretion (158 208 vs 79 176 mg/d, p<0.001) as compared to AGE antibody positive patients. Protein glycation and the ensuing autoimmune response lead to the generation of autoantibodies against AGE. Interactions of AGE autoantibodies with AGE as an antigen being continuously produced result in the formation of AGE-immune complexes that may play a role in the atherogenic processes.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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