Pregled bibliografske jedinice broj: 39829
Diazenes as modificators of intracellular glutathione levels
Diazenes as modificators of intracellular glutathione levels // Kongres hrvatskih biokemičara i molekularnih biologa uz međunarodno sudjelovanje HB 2000 Silver Jubilee Meeting of the Croatian Biochemical Society - Knjiga sažetaka / Floegel, Mirna (ur.).
Zagreb: Jarža d.o.o., 2000. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 39829 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Diazenes as modificators of intracellular glutathione levels
Autori
Moskatelo, Dubravka ; Košmrlj, Janez ; Polanc, Slovenko ; Osmak, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Kongres hrvatskih biokemičara i molekularnih biologa uz međunarodno sudjelovanje
HB 2000 Silver Jubilee Meeting of the Croatian Biochemical Society - Knjiga sažetaka
/ Floegel, Mirna - Zagreb : Jarža d.o.o., 2000
Skup
Kongres hrvatskih biokemičara i molekularnih biologa uz međunarodno sudjelovanje
Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 14.10.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
cell cultures; glutathione; diazene
Sažetak
Glutathione (GSH) is a ubiquitous non-protein thiol essential for cellular homeostasis. It plays an important role in protecting cells against free radical induced oxydant injury, as well as in the detoxification of numerous endogenous and foreign electrophilic compounds. Most studies indicate that modulation of glutathione metabolism may be one of the most promising means of reversing clinical drug-resistance, which is the major obstacle to effective chemotherapy of cancer. Ten new diazenecarboxamides (diazenes) have been synthesized that should, according to their structure and biochemical properties, lower the intracellular GSH content. In the present study we determined intracellular GSH levels in human cervical carcinoma cells following 48 h incubation with equitoxic (the highest non-toxic concentrations) of these diazenes. GSH concentrations were determined spectrophotometrically by the procedure developed by Tietze. The results show that diazene RL-625 lowered significantly the GSH level, diazenes JK-918 and JK-1013 increased significantly the level of GSH, while remaining 7 diazenes (JK-914, UP-11, UP-39, UP-91, RL-338, RL-514 and BR-25) were in this respect inefficient. These results suggest that chemical structure of diazenes is essential for their influence on the intracellular GSH content.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
