Naslov
Isolation and purification of laccase from Trametes vercisolor

Autori
Kovačević, Tatjana, Sirotković, Marina, Tišma, Marina, Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Conference on „ Applied Biocatalysis“ and 5th Meeting of students and university professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2009, 36-37

ISBN
978-961-248-153-7

Skup
1st Conference on „ Applied Biocatalysis“ and 5th Meeting of students and university professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 26.05.2009. - 27.05.2009

Vrsta sudjelovanja
Ostalo

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lacase ; Trametes versicolor ; isolation and purification of laccase ; ultrafiltration ; ion exchange

Sažetak
Abstract: In this work the processes of isolation and purification of laccase from Trametes versicolor were investigated. Fungus was cultivated in the form of mycelial pellets on the complex nutrient media consisted of the waste of pulp and paper industry. After cultivation, pellets were separated from enzyme suspension by the processes of filtration and centrifugation. Concentration of enzyme suspension was performed with ultrafiltration precess using ultrafiltration module (Ultrafiltration cell 8050, Amicon), where the enzyme suspension was concentrated 5 fold. The concentrate was purified using ion exchanger Sephadex G-25. The influence of temperature on efficiency of isolation process and on enzyme activity in the processes of ultrafiltration and ion exchange was investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA