Naslov
Novel Kinetic Spectrophotometric Method for Determination of Tiopronin {; N-(2-Mercaptopropionyl)-glycine};
(Novel Kinetic Spectrophotometric Method for Determination of Tiopronin {; ; ; ; N-(2-Mercaptopropionyl)-glycine}; ; ; ;)

Autori
Kukoč-Modun, Lea ; Radić, Njegomir

Izvornik
Croatica chemica acta (0011-1643) 83 (2010), 2; 189-195

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tiopronin ; N-(2-Mercaptopropionyl)-Glycine ; Kinetic spectrophotometry ; Initial rate method ; Fixed time method ; Pharmaceutical analysis

Sažetak
Novel simple kinetic spectrophotometric method for the determination of tiopronin [N-(2-mercaptopropionyl)-glycine, MPG] in pharmaceutical formulation has been developed and validated. The proposed method is based on the coupled redox-complexation reaction whose first step is reduction of Fe(III) by MPG, while second one includes the complexation of Fe(II), resulted from preceding redox reaction, with 2, 4, 6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)(2)(2+) complex exhibits an absorption maximum at lambda = 593 nm. The initial rate and fixed time (at 3 min) methods were utilized for constructing the calibration graphs. The graphs were linear in concentration ranges from 1.0 x 10(-6) to 1.0 x 10(-4) mol L(-1) for both methods with limits of detection 1.3 x 10(-7) mol L(-7) and 7.5 x 10(-8) mol L(-1) for the initial rate and fixed time method, respectively. The proposed methods were successfully applied for the determination of MPG in its commercial pharmaceutical formulation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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