Naslov
Flow-injection Spectrophotometric Determination of Tiopronin Based on Coupled Redox-Complexation Reaction

Autori
Kukoč-Modun, Lea ; Radić, Njegomir

Izvornik
Chemia analityczna (0009-2223) 54 (2009); 871-882

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
tiopronin; N-(2-Mercaptopropionyl)-glycine; flow-injection spectrophotometry

Sažetak
A flow injection spectrophotometric procedure for determination of N-(2–mercaptopropionyl)- glycine (MPG) tropronin has been proposed. Determination was based on the coupled redox-complexation reaction between MPG, Fe(III) and 2, 4, 6-trypyridyl-S-triazine (TPTZ). Firstly, MPG reduced Fe(III) to Fe(II), which was rapidly converted to the highly stable, deep-blue coloured Fe(TPTZ)2 2+. This coloured complex was monitored at 593 nm. Optimal conditions for determination of MPG were obtained applying the univariate method. A linear calibration curve was established in MPG concentration range 6.0 × 10–6–2.0 × 10–4 mol L–1 with regression equation y = 4675 x – 0.0195 (R2 = 0.9999) (n = 9) and detection limit of 4.0 × 10–6. Analytical frequency was 60 h–1. The proposed method was simple, rapid, sensitive and reproducible (RSD 0.5%, n = 10). It can be applied to determination of up to nanomolar levels of MPG in pharmaceutical preparations. Usual excipients used as additives in pharmaceuticals did not interfere with determination.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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