Naslov
Oxygen influence on amino acid oxidation catalyzed by amino acid oxidase

Autori
Valentović, Ivana ; Findrik, Zvjezdana ; Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Conference on ˝Applied Biocatalysis˝ and 5th meeting of students and university professors from Maribor and Zagreb : Book of Abstracts / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2009, 61-62

ISBN
978-961-248-153-7

Skup
Conference on ˝Applied Biocatalysis˝ (1 ; 2009) ; Meeting of students and university professors from Maribor and Zagreb (5 ; 2009)

Mjesto i datum
Maribor, Slovenija, 26.05.2009. - 27.05.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amino acid oxidase ; racemate

Sažetak
The production of optically pure compounds is one of the most difficult and challenging problems in the field of specialty chemicals today. One of such processes is amino acid racemate resolution catalyzed by amino acid oxidase. Amino acid oxidase catalyzes oxidative deamination of amino acids producing the corresponding  -keto acid. Depending on its stereoselectivity, amino acid oxidase acts on one of the enantiomers, while the other one stays untouched. The system studied in this work is the reaction of D-methionine oxidation. Special attention has been focused on oxygen influence on the reaction rate. Since oxygen is one of the reactants, its concentration in the reaction solution is of great importance. Mathematical model of the process has been developed. It consists of kinetic and mass balance equations in the batch reactor. Kinetics of the reaction was determined by using the initial reaction rate method. The influence of the oxygen and amino acid concentration on the reaction rate was measured. Oxygen volume transfer coefficient has been determined by the independent measurements. The experiments without and with continuous oxygen (air) supply were carried out. Considering that oxygen is spent in the reaction, the reaction is several times faster if it is supplied to the reactor. Nevertheless, in both cases 100 % conversion of substrate was reached.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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