Naslov
Animal waste treatment by protein hydrolysis catalyzed by proteases

Autori
Ana Džaja, Zvjezdana Findrik, Đurđa Vasić-Rački

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Conference on ˝Applied Biocatalysis˝ and 5th meeting of students and university professors from Maribor and Zagreb, Book of Abstracts / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2009, 20-21

ISBN
978-961-248-153-7

Skup
1st Conference on ˝Applied Biocatalysis˝ and 5th meeting of students and university professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 26.05.2009. - 27.05.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
proteases; meat bone meal; hydrolysis

Sažetak
The meat processing industries produce a large amount of solid animal waste. A part of that waste is used for the production of meat bone meal and technical fat, which find their purpose in different areas. Meat bone meal is used as the feeding additive of farm animals (up to 5%), because of its high protein content. However, it can also be hydrolyzed to smaller peptides and amino acids and further transformed in the anaerobic digestion process which produces methane [1] – a valuable energy source. Hydrolysis can be done in alkaline medium, high pressure and temperature or by enzymes. Enzyme hydrolysis of meat bone meal proteins is an attractive alternative to alkaline hydrolysis because the process is carried out at mild conditions (pH and temperature). Enzyme hydrolysis of meat bone meal proteins was investigated in this work. The activities of three different proteases on meat bone meal proteins were examined. The first was chymotrypsin A4 from beef pancreas. The highest acitivity of this enzyme (2499.37 mgprot dm-3 min-1) was obtained at pH 9.0 in Tris-HCl buffer, and at 40 °C. The second protease used was from fungus Aspergillus oryzae. Maximum activity for this enzyme was obtained at pH 6.0 and 30 °C (737.96 mgprot dm-3 min-1). The third protease used was from bacteria Bacillus sp. and it showed no activity on meat bone meal proteins. This enzyme showed no activity on bovine serum albumin as well. Hydrolysis of meat bone meal proteins catalyzed by chymotrypsin A4 and protease from Aspergillus oryzae was carried out in the batch reactor at the optimal conditions for each enzyme. Protein concentration was followed during the experiment. Different amino acids were identified as well. 100 % conversion of proteins was achieved in all experiments.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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