ࡱ> q`bjbjqPqP4:::%NNNNNNNb,,,8J,,bX2v.."......WWWWWWW$Yh'\X-N5..55XNN..GXCCC5T N.N.WC5WCCr`NTNN`O.j. B,,@N@S]X0XN ]B< ]`O`O\ ]NO.0bC23...XXRCd...X5555b&$Jd 'db&J'bbbNNNNNN INFLUENCE OF FUNGICIDES APPLICATION AND SEED PROCESSING TO SUNFLOWER SEED QUALITY `imic Branimir1, Svitlica Brankica3, osic Jasenka2, Andri Luka1, Rozman Vlatka2, Posti Jelena2 , Ivica Liovi1 1 Agricultural institute, Osijek, Ju~no predgrae 17, 31000 Osijek 2 Faculty of Agriculture, Osijek, Trg Sv. Trojstva 3, 31000 Osijek 3 University of Applied Sciences, Po~ega, Pape Ivana Pavla II, br. 6, 34000 Po~ega Corresponding author: dr.sc. Branimir `imic, Agricultural institute Osijek, Ju~no predgrae 17, 31000 Osijek, e-mail:  HYPERLINK "mailto:branimir.simic@poljinos.hr" branimir.simic@poljinos.hr Abstract The aim of the research was to determine influence of methods of seed processing and application of fungicides on mass of 1000 seeds, intergrowth energy and seed germination of sunflower hybrids Favorit and Apolon in 2005 and 2006. Selected untreated seeds of both sunflower hybrids (control) had a minimal mass of 1000 seeds (55-59 g), intergrowth energy (18-37%) and seed germination (39-52%). In both years of research maximal seed quality, mass of 1000 seeds (67-69 g), intergrowth energy (84-85%) and seed germination (87-89%). were after the method of seed processing T-4 and after the fungicides application TF-4. The results showed that unfavorable weather conditions over the production year result with low quality seeds. However, by applying the target methods of seed processing and optimal combination of fungicide doses we can produce the sunflower seeds with regular legislation quality for the market. Key words: sunflower, seed processing, fungicide, treatment, mass of 1000 seeds, intergrowth energy, seed germination. Introduction Sunflower (Helianthus annuus L.) is one of the four most oleigous plants in the world, planted on more than 20 milion hectars, with grain yield from 0.5 to 3.6 tha-1 or averagely 1.16 tha-1 (FAOSTAT Database, 2002). Sunflower yield in Croatia significantly varied over the last 25 years. The average grain yield goes from 1.9 to 2.5 tha-1, depending on the climate factors in the production year (Liovic et al., 2006). With the production of highly qualitative seeds we create basic preconditions for maximal use of cultivar's genetic potential. Basicaly more seeds are produced than what is needed and portion of the seeds produced is kept as a stock. The reason for that is the fact that production agrotecnique and seed yield depend on variety of factors (ecological stress, especially drought). According to the literature the following factors can influence the quality of seeds: climate conditions, pests in the field, oil content of the grain (cultivar's genotype), grain damage in processing, method and duration of grain storage, package, favourable water content in the grain, pesticide effect, storage temeperature, biochemical damage of the tissue of storaged seed (oxidative stress), disease incidence, pests and high oil content in the grain (Andric et al., 2004; Balasevic-Tubic et al. (2005); Hunyadi et al. (2007); Hudec (2006); Simic et al. (2008). All mentioned factors directly and indirectly influence optimal field germination and grain yield. Material and methods Selected natural seeds of sunflower hybrids Apolon and Favorit (natural T-1) were prepared for laboratory examination of the following characteristics: mass of 1000 grains, intergrowth energy and seed germination. After that natural grains were processed: T-2 with round sieves (3.5 mm), T-3 with round sieves 3.5 mm + gravity and T-4 with cut sieves 3.5 mm + gravity. After that the following fungicides were applied on T-4: F1-Vitavax 200FF (karboksin + tiram, 200 ml/100 kg of seeds) + Apron 60DS (metaksil 0.6 kg/100kg of seeds), F2- Vitavax 200FF + Apron 60DS (250 ml/100kg + 0,6kg/100kg of seeds), F3- Vitavax 200FF + Apron 60DS (300 ml/+0,6kg/100 kg of seeds). Seeds samples (F1, F2 and F3) were wet treated in charge sprayer. Intergrowth energy, seed germination and mass of 1000 grains were determined with standard methods (Manual on control of quality of seeds). Obtained values are the average of repetition and statistical work was done with Mstat. Results and discussion Results show that the quality of seeds of sunflower hybrids is in strong correlation to the method of processing and fungicide application (Table 1 and 2). In each year of research the lowest mass of 1000 grains of both hybrids was after selection of the grain (natural grain). The highest mass of 1000 grains of both hybrids was after T-4 processing Apolon 63 g and Favorit 67 g in 2005, and Apolon 59 g and Favorit 69 g in 2006. The difference in the value of 1000 grain mass is significant on the level P<0.05 (Table 1). Intergrowth energy of both hybrids for both years was the lowest at control level (18-39%) and highest with the treatment T-4 (79-80%) and with use of F-3. In both years hybrid Favorit had higher intergrowth energy compared to hyprid Apolon from 7 to 12 %. The difference in the intergrowth energy was reduced with processing for 4 to 8 % and with application of fungicide 3 to 4 % (Table 1 and 2). Seed germination had the same variability as the mass of 1000 grains and intergrowth energy. The highest value was after the treatment T-4 in 2005 for hybrid Favorit and the lowest for hybrid Apolon in 2005 after the grain selection (40%). The significance of the change of the intergrowth energy and seed germination was on the level P<0.01. With the fungicide treatment values of intergrowth energy and seed germination were significantly increased and the mass of 1000 grains was statisticaly insignificantly higher (Table 2). Mass of 1000 grains in both years and with both hybrids was from 60 to 67 g. The increase was a result of processing. After processing and fungicide application the energy of germination was higher for hybrid Favorit in both years (81-86%) with the treatment T-3. Compared to selected (natural) grains treatment T-4 increased the intergrowth energy for 44-61% (Apolon) and from 36 to 59% (Favorit). Seed germination was also significantly higher after the fungicide application T-4 in both research years and with both hybrids. Conclusion Based on the conducted research on the influence of method of processing and fungicide application on the quality of seeds of sunflower hybrids we can conclude the following: natural selected grains of both hybrids had the lowest mass of 1000 grains (55-59 g), intergrowth energy (18-37%) and seed germination (39-52%). Hybrid Apolon in both years had lower seed quality after processing and fungicide application compared to hybrid Favorit after processing mass of 1000 grains (69 g), intergrowth energy (80%) and seed germination (83%) were significantly higher. After fungicide application the quality of seeds of both hybrids was even higher. Obtained research dana shows that even in unfavourable production years high quality of seeds can be reached with appropriate processing and fungicide application. References Andric L., Simic B., Popovic R., Ivanisic I., Plavsic H. (2004). Influence of storage and seed treatment on sunflower seed quality (Croatian). Sjemenarstvo 21(3-4): 143-148. Balesevic-Tubic S., Malencic D., Tatic M., Miladinovic J. (2005). Influence of aging process on biochemical changes in sunflower seed. Helia 28(42): 107-114. Benson E.E. (1990). Free radicals in stressed and aging plant tissue cultures. Plant aging: Basic and applied approaches. 269-275. FAOSTAT. (2002). Food and Agriculture Organization of the UN Database (http://faostat.fao.org/site/567/default.aspx). Hudec K. (2006). Influence of seed treatment, temperature and origin of inocula on pathogenicity of Fusarium species to wheat and barley seedlings. Cereal Res. Comm. 34:1059-1066. Hunyadi Borbely E., Csajbok J., Lesznyak M.. (2007). Relations between the yield of sunflower and the characteristics of the crop year. Cereal Res. Comm. 35:285-288. Krizmanic M., Mijic A., Liovic I., Bilandzic M., Jurkovic Z., and Sudar R. (2004). Realisation of genetic potentials of OS sunflower hybrids in different agroecological conditions. p. 179-180. In: 39th Croatian Symposium on Agriculture, Opatija, Croatia. Liovic I., Kovacevic V., Krizmanic M., Mijic A., Simic B. (2006). Precipitation influence on edible oil production from sunflower in Croatia. Cereal Res. Comm. 34:573-576. Simic B., Cosic J., Liovic I., Krizmanic M., Postic J. (2008): The influence of weather conditions on economic characteristics of sunflower hybrids in macro experiments from 1997 to 2007. 17th International sunflower conference. Cordoba (Spain). 8-12june-2008. p.p. 261-264. Table 1 Influence of the method of processing on the mass of 1000 grains, intergrowth energy and seed germination of sunflower hybrids Hybrid Method of Mass of 1000 Intergrowth energy Seed processing grains germinationApolon 2005 T - 1 55 18 39  T 2 59 66 71 T 3 60 71 77 T 4 63 75 79Favorit T 1 57 25 41 T 2 59 68 72 T 3 62 70 77 T 4 67 79 822006Apolon T 1 57 37 52 T 2 61 60 72 T 3 63 68 78 T 4 65 79 81Favorit T 1 60 49 70 T 2 62 71 78  T 3 63 72 80 T 4 69 80 83 Analysis of variance Source of variation Mass of 1000 grains Intergrowth energy Seed germination F test LSD test F test LSD test F test LSD test 0.05 0.01 0.05 0.01 0.05 0.01Hybrid A n.s. 2.54 3.18 7.632* 11.52 6.15 11.56* 9.78 15.18 Year B n.s. 2.87 3.26 18.567** 9.62 11.57 568.25** 11.64 17.63Treatment C 8.857* 6.72 7.13 678.562** 12.26 14.34 642.15** 6.78 7.56Interaction (ABC) 7.145* 9.17 11.52 358.587** 10.56 12.87 356.478** 4.56 5.17 Table 2 Influence of fungicide application on the intergrowth energy and seed germination of sunflower hybrids Hybrid Method of Mass of 1000 Intergrowth energy Seed processing grains germinationApolon 2005 T - 1 55 20 40  T 2 60 74 78 T 3 60 78 81 T 4 64 81 85Favorit T 1 57 25 41 T 2 58 79 81 T 3 63 80 84 T 4 67 84 872006Apolon T 1 59 39 53 T 2 61 76 79 T 3 64 78 81 T 4 65 83 85Favorit T 1 60 49 70 T 2 62 79 81  T 3 63 82 85 T 4 69 86 89 Analysis of variance Source of variation Mass of 1000 grains Intergrowth energy Seed germination F test LSD test F test LSD test F test LSD test 0.05 0.01 0.05 0.01 0.05 0.01Hybrid A n.s. 5.59 7.24 n.s. 11.52 6.156 14.21* 12.468 21.48 Year B n.s. 3.17 3.51 127.512** 14.87 21.07 724.147** 10.614 11.24Treatment C 74.156** 3,56 4,21 Hnp    & ( , 8 < F H L V Z \ f h j   D F ˿Ӵ˿˿˫Ӵ˿˿ۆ۴ۆh @ h j l    F G H b c d e f n o p ueXNXhk95B*phhk9hk95B*phhVhk9\mHnHsHuhVhk90JmHsH#jhVhk9UmHsHjhVhk9UmHsHhVhk9mHsHhSCcmHsHh MmHsHhk9hk9mHsHhO,mHsHhCJOJQJ\^JaJ h -gh -gCJOJQJ^JaJ h -gh -ghEjICJOJQJ\^JaJh -gCJOJQJ\^JaJ!!!! 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