Naslov
Transition from regional to areal specification of the developing human prefrontal cortex is characterized by the presence of transient belt of MAP2-positive pyramidal cells in the cortical plate.

Autori
Jovanov-Milošević, Nataša ; Petrović, Davor ; Judaš, Miloš ; Kostović, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Forum of European Neuroscience ABSTRACT BOOK / FENS Organization - , 2008

Skup
6th Forum of European Neuroscience

Mjesto i datum
Ženeva, Švicarska, 12.07.2008. - 16.07.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
fetal brain; subplate zone; cortical area

Sažetak
The aim of the present work was to disclose new cytoarchitectonical parameters of developing prefrontal cortex in human, with emphasis on pyramidal projection neurons. We studied 32 specimens from Zagreb Neuroembryological Collection, from 15 postconceptual weeks (PCW) to 13 postnatal months (PM), stained by Nissl method and microtubule-associated-protein-2 (MAP2). First MAP2 reactivity in the cortical plate (CP) was seen as a band below marginal zone at 19 PCW. Between 21 and 26 PCW (when CP displayed incipient lamination), a belt of strongly MAP2-positive pyramidal cells appeared in the middle of the entire prefrontal CP, but was most prominent in dorsolateral prefrontal region. MAP2-reactive cells were also present in the marginal zone, deepest CP (prospective layer VI) and throughout the subplate zone (SP). This belt pattern in CP becomes less prominent at 27 PCW due to the increasing radial and tangential spread of prospective layer V pyramidal neurons and increase in MAP2-staining of other cells in the CP. From 32 PCW to birth, MAP2-positive belt gradually disappeared with concomitant development of areal differences in laminar staining (dorsolateral vs. orbitomedial). At 3 PM, MAP2-positive cells are distributed throughout all cortical layers with remarkable first appearance of MAP2-staining of layer III pyramidal neurons and staining in the remnants of the SP and in gyral white matter. At 13 PM, layer III and V pyramidal neurons displayed similar sizes and MAP2-staining. In conclusion, period between 19-26 PCW is important for cytoskeleton maturation of layer V pyramidal neurons. The transient MAP2-positive belt characterizes developmental transition from regional to areal specification of the prefrontal cortex. This suggests that chemoarchitectonic differentiation of pyramidal neurons precedes the onset of frontal lobe functions. Supported by Croatian Ministry of Science, Education & Sport grant No. 108-1081870-1876 and UKF1B 06/07 (IK).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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