Naslov
Microbiological water quality and environmental impacts of salmon farming

Autori
Kapetanović, Damir ; Vardić, Irena ; Valić, Damir ; Teskeredžić, Zlatica ; Teskeredžić, Emin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Croatian Congress of Microbiology with International Participation-Programme and Abstracts / Abram, Maja ; Ambriović Ristov, Andreja ; Bendelja, Krešo ; Hršak, Dubravka ; Kos, Blaženka ; Kozačinski, Lidija ; Pepeljnjak, Stjepan ; Sviben, Mario ; Vučković, Darinka - Zadar : Hrvatsko mikrobiološko društvo, 2008

ISBN
978-953-96567-7-3

Skup
4th Croatian Congress of Microbiology with International Participation-Programme and Abstracts

Mjesto i datum
Zadar, Hrvatska, 24.09.2008. - 27.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
water quality; microbiology; environmental impacts; salmonids

Sažetak
Bacteria are the most abundant organisms in water. The identification of bacteria which are indicators of pollution is very important in order to carry out monitoring program. The scope of the present research was to analyze environmental impact of intensive salmonide production on water quality parameters during a period of over two years. Sampling locations were determined to include all stages of salmon production: entry to the fish farm, four locations on the farm at different stages of salmon production, outflow from the fish farm, and locations upstream and down stream from the fish farm. Water samples for the heterotrophic bacteria enumeration were serially diluted with Ringer solution pH 6.0 (Pliva) and inoculated by spread plate method on the solid medium according to EN ISO 6222:1999, and incubated at 22°C during 3-5 days. Enumeration of total coliforms and E.coli using Colilert&reg ; , and enterococci using Enterolert&reg ; substrates (IDEXX) were also made. Samples were enumerated by presence in Quanty-Tray/2000&reg ; cells (IDEXX) and data were expressed as the most probable number (MPN) per 100 ml. Colilert test results were read after 24h incubation at 35 &plusmn ; 0.5°C, where yellow wells indicated coliform bacteria, and wells that were yellow and fluorescent when exposed to UV light indicated E.coli. Enterolert tests were incubated for 24h at 41.0&plusmn ; 0.5°C. Test results were read under UV light and fluorescence in a well was considered a positive reaction indicating the presence of enterococci. Data were expressed as the most probable number (MPN) per 100 ml. In general, the waters in the study area were exhibited low bacterial counts for E. coli and enterococci, whereas density of heterotrophic bacteria and total coliforms showed progressive changes which were consistent with nutrient enrichment at different stages of salmon production. The results of these microbiological examination lead to the conclusion that salmonide production has local impact on the water quality.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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