Naslov
Enzymatic transesterification of olive oil and caprylic acid in a solvent system
(Enzymatic transesterification of olive oil and caprily acid in a solvent system)

Autori
Giacometti, Jasminka ; Giacometti, Fabio ; Milin, Čedomila ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
The World Congress on Biotechnology "Biotechnology 2000" and 11th International Biotechnology Symposium and Exibition : proceedings. Vol. 4 / - Frankfurt : Deutsche Gesellschaft für chemisches Apparatewesen (DECHEMA), 2000, 419-421

Skup
The World Congress on Biotechnology "Biotechnology 2000" ; International Biotechnology Symposium and Exibition (11 ; 2000)

Mjesto i datum
Berlin, Njemačka, 03.09.2000. - 08.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
transesterification; solvent system; kinetic model; structured lipids

Sažetak
Structured lipids1-6 (SL) can be defined as triacylglycerols (TAG) restructured or modified to change the fatty acid composition and/or their positional distribution in glycerol molecules by a chemical or enzymatic process. Metabolic pathways and clinical benefits of individual fatty acids that are components of SL are the reason for the synthesis of novel lipid molecules for clinical or nutritional purposes. Lipase-catalyzed transesterification can provide regio- or stereospecific SL. Olive oil is a rich source of monounsaturated fatty acid, oleic acid (MFA) and triolein, too. Transesterification of triolein and caprylic acid was used as a model, because the major TAG part is triolen. SL were prepared by one-step enzymatic transesterification using commercially-available sn-1, 3-specific immobilized lipase (Lipozym) at 50oC in a solvent system (n-hexane) in a batch stirrer-tank reactor for 24 hours at different enzyme and solvent concentrations. The products were taken from the reaction mixture after 2, 4, 6, 12, 24 and 30 hrs and analyzed by reverse-phase high-performance liquid chromatography (HPLC). Acetonitrile/aceton (97/3) was used as the mobile phase and UV detector at 210 nm7. The lipase and solvent concentrations influenced the equilibrium of the transesterification reaction and the composition of the structured lipids. The C37 was higher in triacylglycerols when the concentration of Lipozym was over 24.6 mg/ml as well as when the concentration of n-hexane was over 4.988 (v/v). The C47 was higher in triacylglycerols when the concentration of Lipozym was lower than 24.6 mg/ml and when the concentration of n-hexane was lower than 4.988 (v/v). The low calorie-reduced fats can be synthesized in enzymatic transesterification reaction from olive oil and short- and medium chain fatty acids. References: 1. Lee, K.T. and Akoh, C.C., Food Rev.Int., 14(1) (1998) 17 2. Akoh, C.C.and Huang, K.H., J.Food Lipids 2(1995) 219 3. Fomuso, L.B.and Akoh, C.C., JAOCS 74(3) (1997) 269 4. Lee, K.T. and Akoh, C.C., JAOCS 75(4)(1998) 495 5. Soumanou, M.M., Bornschauer, U.T., Schmid, U. and Schmid, R.D., Fett-Lipid 100(4-5) (1998) 156 6. Soumanou, M.M., Bornschauer, U.T. and Schmid, R.D., JAOCS 75(6) (1998) 703 7. Lee, W.T., JAOCS 63 (3) (1986) 317 8. Basheer, S., Mogi, K. and Nakajima, M., JAOCS 72 (5) (1995) 511 9. Giacometti, J., Academic Dissertation, University of Zagreb, Zagreb 1999.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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