Naslov
Large non homology in heteroduplex DNA is processed differently than single base pair mismatches

Autori
Dohet, Christianne ; Džidić, Senka ; Wagner, Robert ; Radman, Miroslav

Izvornik
MGG. Molecular & general genetics (0026-8925) 206 (1987), 1; 254-256

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
escherichia coli ; bacteriophage ; DNA loops ; DNA repair ; gene conversion

Sažetak
Unmethylated DNA heteroduplexes with a large single stranded loop in one strand have been prepared from separated strands of DNA from two different strains of bacteriophage , one of which has a 800 base pair IS1 insertion in the cI gene. The results of transfections with these heteroduplexes into wild-type and mismatch repair deficient bacteria indicate that such large non- homologies are not repaired by the Escherichia coli mismatch repair system. However, the results do suggest that some process can act to repair such large non-homologies in heteroduplex DNA. Transfections of a series of recombination and excision repair deficient mutants suggest that known excision or recombination repair systems of E. coli are not responsible for the repair. Repair of large non-homologies may play a role in gene conversion involving large insertion or deletion mutations.

Izvorni jezik
Engleski

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