Naslov
Continuous processes with soluble enzymes

Autori
Kragl, Udo ; Vasić-Rački, Đurđa ; Wandrey, Christian

Izvornik
Indian journal of Chemistry. B (0376-4699) 32 (1993); 103-107

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
soluble enzymes; continuous processes

Sažetak
This paper surveys the use of continuously operatingenzyme-membrane reactors with enforced flow where the retentionof soluble enzymes in the reaction vessel is achieved by means ofan ultrafiltration membrane. This technique has beencommercialized in the acylase process for the synthesis ofL-amino acids on a 200 ton/year level. It is especially usefulfor the application of multi-enzyme systems with cofactorregeneration. The synthesis of L-tert-leucine from correspondingà -keto acid has been achieved on a kilogram scale. Coenzymescoupled to water soluble polymers are retained in themembrane-reactor together with the enzymes. Use of suitableconditions prevents loss of enzyme and coenzyme by passagethrough the membrane or by deactivation. Therefore the costs ofenzymes and coenzymes are no longer limitations for economicprocesses. In the continuously operating enzyme-membrane reactor regeneration of the coenzyme up to 600000 times was achieved. Incontinuous peptide synthesis space-time yields 25 kg/(l*d) wereobtained. To suppress side reactions very high catalystconcentrations are possible, yielding residence times below 4min.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA