Naslov
Lipids of the adipose human tissue

Autori
Gall-Palla, Vera ; Šepić Grahovac, Dubravka ; Ružić, A. ; Cerkovnikov, Eugen

Izvornik
Acta Facultatis Medicae Fluminensis (0065-1206) 6-7 (1971), 1-2; 73-79

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lipids; Adipose tissue; Human

Sažetak
Examined were the fat of the kidney capsule and the subcutaneous tissue of 91 patients of different age ( 50-80 years ) and sex. Fat was taken from the autopsy and the surgical operations. The chopped and homogenized adipose tissue was treated with a mixture of chloroform: methanol (2:1) according to Folch. The mild alcalic hydrolysis of the polar lipids isolated the sphingolipids. Different groups of lipids were identified by a thin layer chromatography on the silicagel G with chlorophorm – methanol - water (65:25:4), and phospholipids with chloroform-metanol-7M-ammonia (46:18:3). Adipose human tissue contains 66%-96% of lipids. The main components are nonpolar lipids (95%-99% of the total lipids) and consist of triglycerides and cholesterol. Only 1% of the total lipids are polar: lisolecithin, lisokephalin, lecithin, sulphatide, kephalin, cerebroside, free fat acids and the remaining cholesterol. The slightly increased value for the subcutaneous and kidney fats in women are not significant. The chromatograms on the thin layer silicagel G confirm that there is no difference with regard to sex and the kind of fat in adipose man taken from patients during surgery.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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