Naslov
The activity of extracellular signal-regulated kinase (ERK) is required during G2/M phase before metaphase-anaphase transition in synchronized leukemia cell lines

Autori
Matković, Katarina ; Lukinović-Škudar, Vesna ; Banfić, Hrvoje ; Višnjić, Dora

Izvornik
International Journal of Hematology (0925-5710) 89 (2009), 2; 159-166

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ERK; HL-60; NB4; Cell cycle; Aphidicolin; Nocodazole; G2/M

Sažetak
The pharmacological inhibitors of extracellular signal-regulated kinase (ERK) have been suggested as a novel molecular target-based therapy for acute myeloid leukemia. Several studies have established the role of ERK in cell cycle progression from G1 to S phase in response to mitogen, but the role of ERK after the restriction point is less clarified. In this study, we used models of aphidicolin and nocodazole-synchronized HL-60 and NB4 leukemia cell lines to determine the kinetics of ERK-activity during the progression of the cell cycle and to test the effects of commercially available inhibitors on G2/M progression of synchronized leukemia cells. In aphidicolin-synchronized cells, the activity of ERK was low during early S phase and increased at late S and G2/M phase of the cell cycle. The presence of MEK inhibitors PD 98059 and U0126 caused a delay in G2/M phase. In nocodazole-synchronized cells, the activity of ERK was low during M/G1 transition and MEK inhibitors had no effects on return of the cells to G1 phase. These results demonstrate that the activity of ERK is required during G2/M phase of leukemia cell cycle before the cells reach metaphase-anaphase transition.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA