Naslov
Immunohistochemical analysis of ZAP-70 expression in chronic lymphocytic leukemia

Autori
Korać, Petra ; Ajduković, R. ; Kardum Paro, Mirjana Mariana ; Jakšić, Branimir ; Dominis, Marija

Izvornik
Journal of Molecular Histology (1567-2379) 40 (2009), 1; 81-86

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, stručni

Ključne riječi
CLL; ZAP-70; CD45RO; Immunohistochemistry; Double immunofluorescence; Prognosis

Sažetak
This paper shows a protocol for the detection of ZAP-70 expression in B-CLL (B cell chronic lymphocytic leukemia) tumor cells by common immunohistochemical methods. The study was conducted on bone marrow trephine biopsies from 62 B-CLL patients at the time of diagnosis. Immunohistochemical reactions based on peroxidase and alkaline phosphatase reactions were used, as well as double immunofluorescent labeling for ZAP-70 detection as an indirect marker of mutated and unmutated CLL. Clinical relevance of the ZAP-70 expression detection method was assessed using χ 2 test between ZAP-70 positivity data and other known prognostic factors, i.e., clinical and cytogenetics data. ZAP-70 was detected in 13 out of 62 patients. Statistically significant results were obtained for ZAP-70 positive cases and known indicators of worse prognosis. Immunohistochemical analysis supported by double immunfluorescent labeling, as shown here, is an easy and reliable technique for the detection of ZAP-70 expression in B-CLL tumor cells applicable in every hematopathology laboratory.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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