Naslov
Tumor associated glycoprotein-72 and mucin I show distinct impact on decidual dendritic cells functions

Autori
Redžović, Arnela ; Laškarin, Gordana ; Vlastelić, Ivan ; Solinas, G ; Allavena, Paola ; Mantovani, Alberto ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme and Abstract book / Sozzani, Silvano ; Mantovani, Alberto - Brescia : School of Medicine, University of Brescia, 2008, 88-88

Skup
22nd Annual Meeting Diversity and Plasticity of the Innate Immune Response

Mjesto i datum
Brescia, Italija, 18.09.2008. - 20.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dendritic cells; glycoprotein-72; mucin I; pregnancy

Sažetak
Problem: Possible binding and internalization of endogenous ligands Tumor Associated Glycoprotein-72 (TAG-72) and mucin I (MUC I) by decidual CD1a+ dendritic cells (DC), as well as their influence on DC phenotype, cyto/chemokine production were investigated. Material and methods: Decidual mononuclear cells (DMC) were obtained from the first trimester normal human pregnancy decidua by enzymatic digestion (Collagenase IV) and gradient density centrifugation (Lymphoprep). DMC immediately after isolation were untreated or pretreated with TAG-72, MUC I or mannan for 30 min on ice and then CD209 and CD206 were labeled using antibody directed toward their carbohydrate recognition domains or FITC dextran pinoendocytosis was measured by flow cytometry. Intracellular IFN- , IL-4, IL-10, IL-15 and IL18 cytokines were detected in CD1a+ dendritic cells (DC) from DMC suspensions recovered after 18-hours culture in the medium only or in the presence of TAG-72 or MUC I, respectively. The chemokines CXCL10 and CCL17 were analyzed in TAG-72 or MUC I treated or untreated enriched decidual CD1a+ supernatants by enzyme linked immunosorbent assay (ELISA). Mann-Whitney U test was used to determine statistical significance between two groups of interest. Results: Both, TAG-72 and MUC I, as well as mannan (positive control) significantly reduced anti-CD206 and anti-CD209 mAb binding and FITC dextran endocytosis on a dose dependent manner in early pregnancy decidual CD1a+ cells. TAG-72 decreased CD83 surface expression on CD1a+ cells, whereas MUC I caused up-regulation of IL-1R type II and D6 pro-inflammatory decoy receptors. The addition of TAG-72 in the suspension of DMC minced substantially the percentage of IL-15 and IFN- expressing CD1a+ DC and mean fluorescence intensity (MFI) for IL-15 and IL-18 in CD1a+ cells, whereas such effects were not noticed in MUC I treated cells. Neither TAG-72 nor MUC I affected IL-4 and IL-10 expression in DC. CXCL10 secretion (approximately 40 ng/ml) was four times higher than CCL17 secretion of enriched decidual CD1a+ cells cultured in the medium only and it became balanced in the presence of TAG-72. Conclusions: Decidual CD1a+ cells were able to bind and internalize both endogenous ligands tested by carbohydrate recognition domains of CD206 and CD209 receptors, but their effects differed. TAG-72 efficiently down-regulate Th1 oriented cytokine/chemokine production, whereas MUC I up-regulated pro-inflammatory decoy receptor expression. Acknowledgement: The experiments were financed by EMBIC project European FP6, NoE, LSHM-CT-2004-512040 and Croatian Ministry of Science, Education and Sports (Grants No. 0620402-0376 and No. 0377).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA