Naslov
Immunosuppressive action of cyclosporin on chronic relapsing experimental allergic encephalomyelitis in rat

Autori
Zaputović, Luka ; Rukavina, Danijel

Izvornik
Periodicum Biologorum (0353-9164) 91 (1989), 3; 349-356

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Immunosuppression; Cyclosporin; Chronic relapsing experimental allergic encephalomyelitis; Rat

Sažetak
The prophylactic and therapeutic effect of cyclosporin on chronic relapsing experimental allergic encephalomyelitis (CR-EAE) in rats was investigated. The sensitization of AOxDA)F1 hybrid rats was made by subcutaneous inoculation with 0.1 ml of encephalitogenic emulsion in both back footpads. Animals witch were given cyclosporin orraly in a dose of 20 mg/kg/day during 20 days (starting on the day before sensitisation), did not express any detectable sign of disease. Therapeutic treatment of already developed disease with the same oral dose during 10 days (starting from the firs appearance of symptoms), resulted in rapid regression of clinical signs, although sudden death occurred in 50 % of animals. The effect of prophylactic treatment on the development of the delayed type hypersensitivity (DTH) during the induction phase of disease was followed by skin tests performed on the third, seventh, tenth and fifteenth day after sensitization. While in the control group the delayed type reactivity already existed on the third day after sensitization, increasing up till the ninth day and weakening just at the time of disease appearance, the prophylactic use of cyclosporine inhibited completely the development of DTH reactivity. These results strongly suggest that DTH-cells can play an important role in the pathogenesis of CR-EAE. The beneficial effects of cyclosporine in our model could be explained by its selective activation and/or expansion of T-lymphocyte subsets with different function.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA