Naslov
The effect of MK-801 on the Na+, K+-ATPase activity and ERK activation in rats exposed to global cerebral ischaemia

Autori
Župan, Gordana ; Mršić-Pelčić, Jasenka ; Pelčić, Goran ; Vitezić, Dinko ; Simonić, Ante

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Congress of the Federation of the European Pharmacological Societies (EPHAR) ; u: Fundamental & clinical pharmacology. Vol.18, suppl 1, P12.35 / - , 2004

Skup
4th Congress of the Federation of the European Pharmacological Societies (EPHAR)

Mjesto i datum
Porto, Portugal, 14.07.2004. - 17.07.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
rat; hippocampus; MK-801; Na+; K+-ATPase; ERK; JNK

Sažetak
Aims: We examined the effects of MK-801 on the Na+, K+-ATPase activity and ERK activation in the rat hippocampal tissue after exposure to global cerebral ischaemia of 20 min duration. Procedure: The enzymatic activity and ERK activation were measured at different time points after ischaemia (30 min: 1: 6: 12: 24: 72 and 168 h for the Na+, K+-ATPase activity measurements/5 and 10 min: 1: 6 and 12 h for ERK activation) in control animals such as in the ischaemic animals pretreated with different doses of MK-801 (0.1: 0.3. 1.0 and 3.0 mg/kg), 30 min before exposure to ischaemia. Results: It was found that global cerebral ischaemia induced a statistically significant decrease in Na+, K+-ATPase activity starting from 1 to 168 h of reperfusion. Maximal enzymatic inhibition was obtained 24 h after ischaemic damage. The activation signal of JNK was detected 5 min up to 12 h after ischaemia, but maximal activation was registered after 1 h of reperfusion. Pretreatment with 1.0 or 3.0 mg/kg of MK-801 prevented the decrease of the Na+, K+- ATPase activity and preserved the level of JNK activation in all experimental groups of the ischaemic animals. Conclusions: Decrease in Na+, K+-ATPase activity such as ERK activation are evident during different periods of reperfusion injury after global cerebral ischaemia. Pretreatment with MK-801 prevented changes mentioned above and indicated its possible beneficial role in the treatment of severe brain ischaemia.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA