Naslov
The CR4 region of EBNA2 confers viability of Epstein-Barr virus-transformed B cells by CBF1-independent signalling

Autori
Grabušić, Kristina ; Maier, Sabine ; Hartmann, Andrea ; Mantik, Anja ; Hammerschmidt, Wolfgang ; Kempkes, Bettina

Izvornik
Journal of General Virology (0022-1317) 87 (2006), Part 11; 3169-3176

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Epstein-Barr virus; nuclear antigen 2; B cells; transformation

Sažetak
The Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) gene product is the key regulator of the latent genes of EBV and essential for EBV-mediated transformation of human primary B cells. Viral mutants were constructed carrying a deletion of the EBNA2 conserved region 4 (CR4). Primary resting B cells infected with the DeltaCR4-EBNA2 mutant virus were dramatically impaired for B cell transformation. Lymphoblastoid cell lines (LCLs) established with this mutant EBV revealed a prolonged population doubling time when cells were cultivated at low cell densities, which are not critical for wild-type-infected cells. Low-level spontaneous cell death occurred when the cells were cultivated at suboptimal cell densities. The phenotype of B cells and LCLs infected with the DeltaCR4-EBNA2 mutant virus indicated that the CR4 region of EBNA2 specifically contributes to the viability of the cells rather than affecting cell division rates.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA