Naslov
Cathepsin D and plasminogen activator inhibitor type 1 in normal, benign and malignant ovarian tissues: a preliminary report

Autori
Šprem, Marina ; Babić, Damir ; Abramić, Marija ; Miličić, Duško ; Vrhovec, Ivan ; Škrk, Janez ; Osmak, Maja

Izvornik
Radiology and oncology (1318-2099) 34 (2000), 1; 41-47

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ovarian neoplasms; cathepsin D; plasminogen activator inhibitor type 1

Sažetak
Background. The aim of the present study was to determine the concentration of cathepsin D (Cath D) and plasminogen activator inhibitor type 1 (PAI-1) in normal ovarian tissue, benign and malignant ovarian tumor tissues and to asses relationship between Cath D and PAI-1 content, and some clinical and pathohistological parameters. Materials and methods. Cath D contents and PAI-1 concentrations were determined (using immunoradiometric ELSA-Cath D assay and commercial IMUDINDR ELISA immunoassay, respectively) in 35 samples: 10 normal ovarii, 10 benign, 10 primary malignant and 5 metastatic ovarian tumors. Results. The concentrations of Cath D were significantly higher in malignant (32.89ą14.26 pmol/mg protein ) and metastatic (31.42 ą10.24 pmol/mg protein) than in normal (13.68ą4.03 pmol/mg protein) and benign (17.89ą13.13 pmol/mg protein) ovarian tissues. There was no statistical differences in the concentrations of PAI-1 between normal, benign, malignant and metastatic tumor specimens. The concentration of cathepsin D as well as PAI-1 did not correlated to age of patients, menopausal status, parity, GOG risk group, clinical stage or pathohistological grading. Conclusion. Concentrations of Cath D ( but not PAI-1) were significantly increased in malignant ovarian tumor tissues when compared to normal and benign ovarian tumor samples; they were independent from examined pathohistological and clinical parameters.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA