Naslov
SPE-HPLC/DAD determination of trimethoprim, oxytetracycline and enrofloxacin in water samples

Autori
Ašperger, Danijela ; Babić, Sandra ; Mutavdžić Pavlović, Dragana ; Dolar, Davor ; Košutić, Krešimir ; Horvat, Alka J. M. ; Kaštelan-Macan, Marija

Izvornik
International journal of environmental analytical chemistry (0306-7319) 89 (2009), 8-12; 809-819

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
veterinary antibiotics ; trimethoprim ; oxytetracycline ; enrofloxacin ; water ; solid-phase extraction (SPE) ; high-performance liquid chromatography (HPLC) ; membrane treatment

Sažetak
In this paper high performance liquid chromatography with diode array UV detection (HPLC/DAD) after SPE sample pretreatment for simultaneous analysis of pharmaceuticals from three different classes: enrofloxacin, oxytetracycline and trimethoprim has been developed, optimised and validated. The chromatographic separation was developed on spiked wellspring water samples and cheeked on model wastewater samples of veterinary pharmaceuticals before and after RO/NF membrane treatment (feed and permeate streams) and on process wastewaters of industrial origin. Chromatographic separation was performed on Varian ProStar HPLC/DAD with C-18 column (Microsorb-MV 100 C18, 150x4.6 mm ; 5 µ ; ; ; ; m, Varian, USA). Detection and quantification was performed at 254 nm. The best separation was achieved with mobile phase 0.5 % formic acid and 1 % trifluoroacetic acid in 0.05 M ammonium acetate-methanol, 70+30, (v/v) after extraction procedure on polystyrenedivinylbenzene Varian Empore extraction disks. The extraction efficiency was checked by recovery experiments.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne tehničke znanosti

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