Naslov
A simple and reliable method for monitoring platelet serotonin levels in rats

Autori
Jernej, Branimir ; Čičin-Šain, Lipa ; Iskrić, Sonja

Izvornik
Life Sciences (0024-3205) 43 (1988), 20; 1663-1670

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
serotonin ; platelet ; rat

Sažetak
A simple and reliable method for individual monitoring of platelet serotonin in rats is developed. Platelet-rich plasma is prepared under standardized conditions from 1 mL of venous blood and the platelets are quantitatively separated by a highly reproducible procedure. Platelet serotonin content is determined spectrophotofluorometrically and the results are comparatively expressed per standardized platelet rich plasma sample (1.01 ± ; 0.18 μ g), per mg of platelet protein (1.57 ± ; 0.15 μ g) and per 109 platelets (2.16 ± ; 0.38 μ g). Normal distribution of platelet serotonin levels in a sample of 338 animals is shown. By use of the described method, the intraindividual stability of platelet serotonin concentration in rats is demonstrated for the first time.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA