Introduction of the gene amplification technology to decrease the risk of hepatitis C virus transmission by plasma products

Branović, Karmen; Forčić, Dubravko; Košutić-Gulija, Tanja; Šantak, Maja; Zgorelec, Renata; Mažuran, Renata

Pregled bibliografske jedinice broj: 38156

Introduction of the gene amplification technology to decrease the risk of hepatitis C virus transmission by plasma products

Branović, Karmen; Forčić, Dubravko; Košutić-Gulija, Tanja; Šantak, Maja; Zgorelec, Renata; Mažuran, Renata

Introduction of the gene amplification technology to decrease the risk of hepatitis C virus transmission by plasma products // Journal of Chromatography A, 852 (1999), 1; 305-312 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 38156 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Introduction of the gene amplification technology to decrease the risk of hepatitis C virus transmission by plasma products

Autori
Branović, Karmen ; Forčić, Dubravko ; Košutić-Gulija, Tanja ; Šantak, Maja ; Zgorelec, Renata ; Mažuran, Renata

Izvornik
Journal of Chromatography A (0021-9673) 852 (1999), 1; 305-312

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hepatitis C virus; viruses; RNA

Sažetak
The viral safety of plasma-derived products with respect to hepatitis C virus (HCV) is assured by selection of donors, screening of individual donations for antibodies to HCV and the incorporation of effective viral inactivation-removal steps into manufacturing processes. As antibody screening of single donations is not sufficient to completely eliminate HCV RNA positive plasmas from plasma pools, testing for HCV RNA by gene amplification techniques may be necessary to identify positive donors. Using modern molecular biology techniques, we developed a specific, sensitive and reproducible method for routine PCR screening for HCV RNA in plasma pools.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
021001

Ustanove:
Imunološki zavod d.d.

Profili:

Maja Šantak (autor)