Naslov
CAST-ELISA Test in the Diagnosis of Allergy to Aditives

Autori
Turčić, Petra ; Rudolf, Maja ; Milavec-Puretić, Višnja ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the IXth World Conference on Clinical Pharmacology and Therapeutics ; u: Canadian Journal of Clinical Pharmacology 15 (2008) (3S) e420-e781 ; From Fundamental to Clinical Pharmacology, 615 / Canadian Society for Pharmacology and Therapeutics - Quebec, 2008, E709-e709

Skup
World Conference on Clinical Pharmacology and Therapeutics (9 ; 2008)

Mjesto i datum
Quebec, Kanada, 27.07.2008. - 01.08.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
allergy; aditives; CAST-ELISA

Sažetak
Allergy to additives can be in combination with food and drug allergy serious diagnostic and therapeutic problem. The mechanisms by which food additives affect the immune mechanisms are poorly understood. These reactions are described as intolerance or pseudoallergy and are not IgE-mediated. Concomitant drug allergy to vehicles must be diagnosed too. At present, detection of food additives allergy is still difficult and requires an adequate clinical examination and specific in vivo and in vitro diagnostic tests. The aim of the study was to investigate the diagnostic value of cellular antigen stimulation test (CAST) in detection of hypersensitivity reaction to additives. We tested 47 patients with positive history of hypersensitivity reactions to food additives (sodium salicylate, sodium benzoate, potassium metabisulfite and tartrazine). Clinical symptoms included urticaria/angioedema (25 patients), medicamentous exanthema (6 patients) and eczematous dermatitis (16 patients). Sulfidoleukotrienes (sLT) quantification was carried out by CAST-ELISA after in vitro peripheral blood leukocytes stimulation with food additives. The results higher than the cut-off range value (sodium salicylate 120 pg/mL, sodium benzoate. 90 pg/mL, potassium metabisulfite 40 pg/mL and tartrazine 120 pg/ml) were considered positive. Our results demonstrate that CAST was positive in 8% (4/45) of patients with allergy to sodium salicylate, 26% (12/45) of patients to sodium benzoate, 28% (13/37) of patients to potassium metabisulfite and 28 % (13/45) of patients to tartrazine. Our results show that increased sulfidoleukotriene production was observed with different frequency by any off the food additive tested. In vitro measurement of sLT production may be helpful or identifying some patients with allergy to food additive and drug allergy.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA