Naslov
Effect of extracellular NADH on human tumor cell proliferation

Autori
Slade, Neda ; Štorga-Tomić, Danijela ; Birkmayer, George D. ; Pavelić, Krešimir ; Pavelić, Jasminka

Izvornik
Anticancer research (0250-7005) 19 (1999); 5355-5360

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NADH; tumor; proliferation

Sažetak
We investigated the antiproliferative effects of extracellular mcotinamide adenine dinucleotide against human malignant CaCo-2 (colon carcinoma), Hep-2 (laringeal carcinoma) cell lines, as well as against murine fibrosarcoma and normal human embrional fibroblast (HEF). NADH was very potent in the growth inhibition of murine fibrosarcoma and human Hep-2 cells, regardless of the dose applied. During the observed period (4 or 5 days) only one dose of NADH was sufficient in reducing the growth rate for up to 92%. It had no effect on the growth of other cell lines tested. The identification of DNA-fragmentation and p53 and Ki-67 genes expression suggest that the mechanism of NADH action is different from disregulation of genes considered as checkpoints in cell cycle.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA