Naslov
Effects of pioglitazone on the lipid peroxidation levels and COX-2 expressions in different brain structures of rats with lithium plus pilocarpine- induced status epilepticus

Autori
Župan, Gordana ; Peternel, Sandra ; Pilipović, Kristina ; Blagaić, Ana ; Hrelja, Ana ; Vitezić, Dinko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 12th Congress of the European Federation of Neurological Societies ; u: European Journal of Neurology. Volume 15 (2008) (S3) / Lenzi, Gian Luigi ; Gil-Nagel, Antonio - Madrid : Wiley-Blackwell, 2008, 307-307

Skup
Congress of the European Federation of Neurological Societies (12)

Mjesto i datum
Madrid, Španjolska, 23.08.2008. - 26.08.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
status epilepticus; oxidative stress; cyclooxygenase-2; pioglitazone; rats

Sažetak
In our previous study we demonstrated that pioglitazone, a synthetic agonist of the peroxisome proliferator-activated receptors (PPAR) gamma, causes the prominent increase of the 24 h- survival rate in rats with lithium plus pilocarpine-induced status epilepticus (SE), that provides a common experimental model of temporal lobe epilepsy (TLE) in humans. With the aim to investigate possible mechanisms underlying mentioned drug effect, we determined the lipid peroxidation levels and COX-2 expressions in some brain structures of rats with SE and treated by pioglitazone. Experiments were performed on 80 to 90 days old male Wistar rats. Pioglitazone (1 ; 3 mg/kg) or vehicle were administered i.p. 10 min after the lithium plus pilocarpine-induced SE, that was interrupted 2 h after its onset by an injection of diazepam. Rats were sacrificed 24 h after the SE onset and Western blotting analyses of COX-2 expressions were performed in the piriform/entorhinal cortex, temporal neocortex and in the hippocampus. Levels of the brain lipid peroxidation were determined by thiobarbituric acid reactive substances (TBARS) assay. Lithium plus pilocarpine-induced SE caused increases in the lipid peroxidation levels and COX-2 overexpressions in all examined brain regions. In rats with SE and treated by pioglitazone, TBARS levels were reduced in the piriform/entorhinal cortex and in the temporal neocortex. COX-2 overexpression was decreased in the hippocampus. Conclusion: Our results suggest that pioglitazone could be a candidate drug to protect examined brain structures in patients with TLE.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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