Naslov
Application of molecular methods in soybean breeding program at the Agricultural Institute Osijek

Autori
Sudarić, Aleksandra ; Vratarić, Marija ; Duvnjak, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - Poljoprivreda (Agriculture, Vol.16, Num. 1, Supplement) / Jurković, Draženka ; Šimić, Domagoj - Osijek : Pannonian Plant Biotechnology Association ; Agricultural Institute Osijek, 2008, 20-20

Skup
Plant Breeding and Biotechnology in the Great Pannonian Region - Present and Future Needs

Mjesto i datum
Osijek, Hrvatska; Beli Manastir, Hrvatska, 07.04.2008. - 09.04.2008

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
soybean; breeding; molecular marker

Sažetak
The soybean breeding work at the Agricultural Institute Osijek has focused on the permanently development of high-yielding cultivars with genetic yield potential of 5-6 t/ha, satisfactory grain quality (protein and oil content), high tolerance to the principal diseases (Peronospora manshurica, Sclerotinia sclerotiorum, Diaporthe/Phomopsis complex), high resistance to lodging, stress conditions over vegetation and pod shatering as well as satisfactory stability in level and quality of grain and wide adaptability. Results of this continued and intensive breeding work are 36 registered cultivars which significantly contributed and contribute to the development, improving and increasing of soybean production in Republic of Croatia. Further genetic improvement of soybean cultivars is based on the modern breeding strategies including combination of conventional breeding methods and recent chemical, biochemical, fitopathology and molecular analyses. Regarding to molecular analyses, in recent years, in the frame of the soybean breeding program has initiated by application of molecular markers technology as criterion for estimation genetic diversity for both soybean germplasm and pathogens from Diaporthe/Phomopsis complex on soybean, as well. The initial fingerprinting of several OS soybean genotypes has performed in collaboration with the University of Guelph (Canada) in their biomolecular laboratory using simple sequence repeats (SSR). The obtained results enabled new access in choosing parental pairs. Combining molecular markers technique with pedigree information, phenotypic markers and statistical procedure has provided a useful tool for more accurate and complete evaluation of genetic diversity and its more effective utilisation into current soybean breeding program. The detecion of pathogens from Diaporthe/Phomopsis complex on soybean on molecular level has performed in collaboration with the Istituto Sperimentale per la Patologia Vegetale (Rome, Italy) in their micological and biomolecular laboratories using method of artificial infection and RFLP markers. Obtained data are incorporated in our breeding work for improving soybean genotypes tolerance on metioned pathogens. In a whole, implementation of molecular marker technology into soybean breeding program at the Institute represents basis for significantly increasing of its quality, sucess, efficiency and competitivenes and enables further genetic improvement of cultivars. Each increasing of soybean production, resulting from genetic improved cultivars, is considerable national economical profit.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA