Naslov
Calcitonin gene-related peptide receptors in pig spleen and involvement of the CGRP1 receptor in the splenocyte function

Autori
Haberstock-Debić, Helena ; Weyns, A.A. ; Marotti, Tanja ; De Potter, W.

Izvornik
Neuropeptides (0143-4179) 33 (1999), 1; 47-53

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
Calcitonin gene-related peptide (CGRP)-positive nerve fibers have been found in the trabecula and parenchyme area of pig spleen. Receptor studies have demonstrated that the CGRP binding site in pig spleen membranes has an average Kd 2, 24 +/- 0, 48 nM and Bmax 78 +/- 4, 09 fmol/mg of protein. In the Kd range demonstrated in the binding studies, the dose-dependent suppressive effect of CGRP on spleen T lymphocyte proliferation was found with the maximal effect in 10-9 M concentration. The same effect, but in a different concentration, was found on peripheral blood T lymphocytes with the maximum in 10-6 M concentration. Contrary to the results obtained through the simultaneous presence of CGRP and mitogen, preincubation with CGRP led to a stimulation of peripheral blood lymphocytes in response to ConA and had no effect on spleen T lymphocytes. These results illustrate the difference in CGRP effect between lymphocytes of different origins. Using CGRP1 receptor antagonist CGRP8-37, we established that the CGRP suppressive effect on spleen T lymphocyte proliferation is CGRP1-receptor mediated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA