Naslov
Growth and metabolic characteristics of CCO cells in static and dynamic cultivation systems

Autori
Slivac, Igor ; Radošević, Kristina ; Gaurina Srček, Višnja ; Fumić, Ksenija ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 13th International Biotechnology Symposium & Exhibition / Zhang, Wei ; Bai, Fengwu ; Zhong, Jian-jiang ; Yang, Shengli - Dalian : Elsevier, 2008, 135-135

Skup
13th International Biotechnology Symposium & Exhibition

Mjesto i datum
Dalian, Kina, 12.10.2008. - 17.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CCO; cell metabolism; Wave bioreactor; microcarriers

Sažetak
The cultivation of animal cells is of great importance for effective and reasonable bioprocess production of various therapeutic proteins as well as for fundamental laboratory research. The metabolic patterns of animal cells are altered substantially during the actions of establishment of a typical cell line designed for in vitro techniques. Therefore, characterization and understanding of cell metabolism are needed to improve the processes with cultivated animal cells. The channel catfish ovary cell line (CCO) was derived from the ovaries of a juvenile channel catfish some thirty years ago. Since then, it has been used for channel catfish virus vaccine production and related in vitro laboratory research. However there is still no enough data for basal understanding of their metabolism. We report on growth characteristics of CCO cells cultivated in multiwell plates and Wave bioreactor. Specific growth rate of CCO cells in multiwell plates was 0, 0206 h-1. The concentration of glucose in culture media remained unchanged through whole cultivation period. Glutamine was almost completely depleted and ammonia, its major wasteproduct, reached concentration of 2, 3 mM. Amino acid analysis of culture media showed that highly consumed amino acids, besides glutamine, were arginine, serine, and threonine, suggesting that they were main energy source. Cytodex 1 microcarriers were used in order to investigate medium-scale cultivation parameters of CCO cells in the Wave bioreactor. In this cultivation system oxygen transfer and mixing are accomplished by the wave-induced agitation. Micocarrier confluence was reached with cell density of 1, 8 x 10e6 cells/mL and cell growth rate of 0, 0182 h-1. The results proved the feasibility of employed culture conditions for cultivation of CCO cells.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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