Naslov
Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line

Autori
Gaurina Srček, Višnja ; Radošević, Kristina ; Kniewald, Hrvoje ; Slivac, Igor ; Kmetič, Ivana ; Kniewald, Zlatko

Izvornik
In vitro cellular & developmental biology. Animal (1071-2690) 45 (2009), 1/2; 28-31

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prikaz, znanstveni

Ključne riječi
BHK-21 [C-13] cells; cell proliferation; basic fibroblast growth factor; porcine brain extract; primary cell cultures

Sažetak
Growth factors from neural tissues have been described as potent mitogens for a wide variety of mesoderm- and ectoderm-derived cells in vitro. We used porcine brain extract for in vitro testing of proliferation properties on primary ovarian cells, uterine cells, and cardiomyocytes in culture as well as for BHK-21 [C-13] cell line. The addition of this extract accelerates proliferation in all examined cultures. It also lowers serum requirement and shortens the cultivation period for BHK-21 [C-13] cells. Fibroblast growth factors from brain of different species, but not porcine, are already characterized and their proliferative effect proved. Therefore, we purified, determined, and confirmed the presence of basic fibroblast growth factor in porcine brain extract by Western blot analysis and showed its biological activity on BHK-21 [C-13] cells.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Biotehnologija

POVEZANOST RADA