Naslov
Assessment of percutaneous absorption of 2-butoxyethanol by microdialysis in volunteers

Autori
Korinth, Gintautas ; Wellner, Tanja ; Jakaša, Ivone ; Kežić, Sanja ; Kruse, Jacob ; Schaller, Karl H.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th International Conference Perspectives in percutaneous penetration : abstracts / - , 2004

Skup
International Conference Perspectives in percutaneous penetration (9 ; 2004)

Mjesto i datum
La Grande-Motte, Francuska, 13.04.2004. - 17.04.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microdialysis; butoxyethanol; biological monitoring; percutaneous absorption

Sažetak
The assessment of the penetration kinetics of 2-butoxyethanol (BE) in healthy volunteers using microdialysis technique. 4 male volunteers were exposed on the left forearm to both 90% and 50% v/v aqueous solution of 2-BE for 4.5 hours. The exposed skin area was 0.64 cm2. BE was applied under occlusion and the volume of the BE test solution was 200 µ ; l/cm2. As receptor fluid in the microdialysis capillaries, saline solution was used. To find out the intra-individual and inter-individual variation of percutaneous absorption, each experiment was performed in duplicate on separate days. The percutaneous absorption kinetics was assessed by the measurement of BE in the dialysate samples collected every 30 min for 4 hours. The internal exposure, and the recovery of the BE in the dialysate was assessed from the creatinine adjusted concentrations of the free butoxyacetic acid (BAA) in urine collected over 4.5 hours after starting of the exposure. Maximum penetration rates for both 2-BE dilutions were reached in 6 of 8 experiments after 2-3 h of exposure. There was relatively high intra- and inter-individual variation in the cumulative amount of BE recovered in the dialysate. The cumulative amount of BE after exposure to 50% BE amounted to 30.2 µg (SD 24.6), and after exposure to 90% BE the cumulative amount was 14.0 µg (SD 11.7). The average concentration of the excreted free BAA was 3.46 mmol BAA/mol creatinine. By using values from the literature for the excretion of free BAA after inhalation exposure of a known dose, we calculated the average dermal fluxes of 0.91 (SD 0.37) and 0.56 (SD 0.58) mg cm-2hr-1 for 50% and 90% BE, respectively. The BE amount recovered in the microdialysis tubing was much lower than the amount absorbed into the systemic circulation. The microdialysis technique offers the possibility for studying the in vivo dermal absorption kinetics and metabolism withouth interference. However, the main problem of microdialysis remains the calibration of the technique i.e., the assessment of the relative recovery. The recovery may be determined using parallel in vitro approach. In this study we have determined the recovery of BE in the microdialysate using biological monitoring. The results have shown that only a few percent of the total amount penetrated the skin was recovered in the dialysate. The higher penetration rates of 50% BE in comparison with 90% BE for a factor of 1.6 was in accordance with the values given in the literature.

Izvorni jezik
Engleski

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